RasGRP4 Antibody (E-2): sc-515508

RasGRP4 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RasGRP4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RasGRP4 antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RasGRP4, or Ras guanyl releasing protein 4, is a crucial member of the RasGRP family of guanine nucleotide-releasing factors that play a significant role in activating Ras proteins in mammalian cells, particularly within hematopoietic tissues. RasGRP4 is predominantly expressed in mast cells and is essential for various cellular processes, including cell signaling and proliferation. RasGRP4 is localized in the cytoplasm and features a unique structure that includes one EF-hand domain for calcium binding, an N-terminal Ras-GEF domain, and a phorbol-ester/DAG-type zinc finger that interacts with diacylglycerol (DAG). RasGRP4′s ability to bind DAG regulates recruitment to the plasma membrane, where RasGRP4 is involved in membrane ruffling and the spreading of activated cells. Dysregulation or mutations in the gene encoding RasGRP4 have been linked to increased susceptibility to conditions such as asthma and certain cancers, highlighting RasGRP4′s significance in both normal physiology and disease states.

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RasGRP4 Antibody (E-2) References:

1. RasGRP4 is a novel Ras activator isolated from acute myeloid leukemia.  |  Reuther, GW., et al. 2002. J Biol Chem. 277: 30508-14. PMID: 11880369
2. RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function.  |  Yang, Y., et al. 2002. J Biol Chem. 277: 25756-74. PMID: 11956218
3. RasGRP4 regulates the expression of prostaglandin D2 in human and rat mast cell lines.  |  Li, L., et al. 2003. J Biol Chem. 278: 4725-9. PMID: 12493770
4. Mast cells in airway hyporesponsive C3H/HeJ mice express a unique isoform of the signaling protein Ras guanine nucleotide releasing protein 4 that is unresponsive to diacylglycerol and phorbol esters.  |  Li, L., et al. 2003. J Immunol. 171: 390-7. PMID: 12817022
5. Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells.  |  Zheng, Y., et al. 2005. Blood. 105: 3648-54. PMID: 15657177
6. RasGRP4 in mast cell signalling and disease susceptibility.  |  Stevens, RL., et al. 2005. Novartis Found Symp. 271: 54-68; discussion 68-77, 95-9. PMID: 16605128
7. The Diacylglycerol-dependent translocation of ras guanine nucleotide-releasing protein 4 inside a human mast cell line results in substantial phenotypic changes, including expression of interleukin 13 receptor alpha2.  |  Katsoulotos, GP., et al. 2008. J Biol Chem. 283: 1610-1621. PMID: 18024961
8. Galectin-3 regulates RasGRP4-mediated activation of N-Ras and H-Ras.  |  Shalom-Feuerstein, R., et al. 2008. Biochim Biophys Acta. 1783: 985-93. PMID: 18413234

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RasGRP4 Antibody (E-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RasGRP4 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RasGRP4 Antibody (E-2) Specifications

Alternate Product Name: RasGRP4

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 238-256

Epitope Target: RasGRP4

Epitope Species: human

Product Reactivity/Detection: RasGRP4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RasGRP4 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin