RasGRP1 Antibody (199): sc-8430
- RasGRP1 Antibody (199) is a mouse monoclonal IgG1 κ RasGRP1 antibody, cited in 27 publications, provided at 200 µg/ml
- raised against full length RasGRP1 of rat origin
- RasGRP1 Antibody (199) is recommended for detection of RasGRP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RasGRP1 Antibody (199) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RasGRP1 Antibody (199) for WB applications. These reagents are now offered in bundles with RasGRP1 Antibody (199) (see ordering information below).
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RasGRP Antibody (199) is a mouse monoclonal IgG1 kappa light chain antibody that detects RasGRP of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RasGRP antibody (199) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RasGRP plays a crucial role in cellular signaling as a guanine nucleotide-releasing factor for Ras, which is a key player in the regulation of the MAP kinase pathway. This pathway is essential for various cellular processes, including growth, differentiation, and survival. RasGRP contains two EF hand domains that bind calcium ions and a diacylglycerol (DAG)-binding domain, allowing RasGRP to integrate signals from both calcium and DAG, which are important for Ras activation. The expression of RasGRP in the nervous system and lymphoid tissues highlights RasGRP′s significance in linking extracellular signals to intracellular responses, making RasGRP a vital component in the modulation of immune responses and neuronal signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RasGRP1 Antibody (199) References:
- The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. | Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
- The function of GRB2 in linking the insulin receptor to Ras signaling pathways. | Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
- Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1. | Zhang, XF., et al. 1993. Nature. 364: 308-13. PMID: 8332187
- Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2. | Chardin, P., et al. 1993. Science. 260: 1338-43. PMID: 8493579
- RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. | Ebinu, JO., et al. 1998. Science. 280: 1082-6. PMID: 9582122
- Regulation of RasGRP via a phorbol ester-responsive C1 domain. | Tognon, CE., et al. 1998. Mol Cell Biol. 18: 6995-7008. PMID: 9819387
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RasGRP1 Antibody (199) | sc-8430 | 200 µg/ml | $316.00 | |||
RasGRP1 Antibody (199): m-IgG Fc BP-HRP Bundle | sc-528232 | 200 µg Ab; 10 µg BP | $354.00 | |||
RasGRP1 Antibody (199): m-IgGκ BP-HRP Bundle | sc-520558 | 200 µg Ab, 40 µg BP | $354.00 | |||
RasGRP1 Antibody (199): m-IgG1 BP-HRP Bundle | sc-542829 | 200 µg Ab; 20 µg BP | $354.00 | |||
RasGRP1 Antibody (199) AC | sc-8430 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RasGRP1 Antibody (199) HRP | sc-8430 HRP | 200 µg/ml | $316.00 | |||
RasGRP1 Antibody (199) FITC | sc-8430 FITC | 200 µg/ml | $330.00 | |||
RasGRP1 Antibody (199) PE | sc-8430 PE | 200 µg/ml | $343.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 488 | sc-8430 AF488 | 200 µg/ml | $357.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 546 | sc-8430 AF546 | 200 µg/ml | $357.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 594 | sc-8430 AF594 | 200 µg/ml | $357.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 647 | sc-8430 AF647 | 200 µg/ml | $357.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 680 | sc-8430 AF680 | 200 µg/ml | $357.00 | |||
RasGRP1 Antibody (199) Alexa Fluor® 790 | sc-8430 AF790 | 200 µg/ml | $357.00 |