RASAL1 CRISPR/Cas9 KO Plasmid (m): sc-422603
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RASAL1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RASAL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RASAL1 Antibody (B-2): sc-398025
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RASAL1 CRISPR/Cas9 KO Plasmid (m) | sc-422603 | 20 µg | $397.00 |
Overview
Rasal1 encodes RASAL1, a Ras GTPase-activating protein that accelerates the conversion of active Ras-GTP to inactive Ras-GDP, thereby restraining Ras-driven signaling. By modulating downstream RAF–MEK–ERK and PI3K–AKT pathway activity, RASAL1 influences cell proliferation, survival, and differentiation programs, including context-dependent effects on epithelial and mesenchymal phenotypes. Altered RASAL1 function or expression has been associated with dysregulated growth control and fibrotic remodeling in multiple tissues, supporting its relevance to studies of signaling homeostasis and disease-associated cellular reprogramming in mouse models. These properties make Rasal1 a useful node for dissecting Ras pathway feedback and cross-talk with transcriptional and extracellular matrix regulatory networks.
RASAL1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rasal1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rasal1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rasal1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RASAL1 protein expression.
This CRISPR knockout system enables efficient generation of Rasal1-deficient cell models for investigation of RASAL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rasal1 exon(s) critical for RASAL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rasal1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RASAL1 CRISPR/Cas9 KO Plasmid (m) and RASAL1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rasal1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RASAL1 HDR Plasmid (m) and RASAL1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rasal1 homology arms to support homology-directed repair at defined Rasal1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.