Ras GAP Antibody (D4B4): sc-64
- Ras GAP Antibody (D4B4) is a mouse monoclonal IgG1, cited in 2 publications, provided at 200 µg/ml
- raised against GAP protein
- recommended for detection of of human origin by WB and IP
- See Ras GAP (B4F8): sc-63 for Ras GAP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ras GAP Antibody (D4B4). This work is in progress.
Ras GAP Antibody (D4B4) is a mouse monoclonal IgG1 antibody that detects Ras GAP in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Ras GAP, or GTPase-activating protein, plays a crucial role in regulating the activity of the p21Ras protein, which is a key player in cell signaling pathways that control cell growth and differentiation. By promoting the hydrolysis of GTP to GDP, Ras GAP effectively turns off the signaling of p21Ras, preventing uncontrolled cell proliferation that can lead to cancer. The importance of Ras GAP in maintaining cellular homeostasis cannot be overstated, as mutations or dysregulation of this protein can contribute to oncogenic processes. Ras GAP interacts with p21Ras to enhance its GTPase activity, and dysfunction is often implicated in various malignancies, making Ras GAP a significant target for therapeutic intervention. Anti-Ras GAP antibody (D4B4) is an essential tool for researchers studying the intricate mechanisms of Ras signaling and its implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ras GAP Antibody (D4B4) References:
- Role of p120 Ras-GAP in directed cell movement. | Kulkarni, SV., et al. 2000. J Cell Biol. 149: 457-70. PMID: 10769036
- Semaphorin 4D/Plexin-B1-mediated R-Ras GAP activity inhibits cell migration by regulating beta(1) integrin activity. | Oinuma, I., et al. 2006. J Cell Biol. 173: 601-13. PMID: 16702230
- GTP hydrolysis mechanisms in ras p21 and in the ras-GAP complex studied by fluorescence measurements on tryptophan mutants. | Antonny, B., et al. 1991. Biochemistry. 30: 8287-95. PMID: 1883817
- Regulation of Ras-GAP and the neurofibromatosis-1 gene product by eicosanoids. | Han, JW., et al. 1991. Science. 252: 576-9. PMID: 1902323
- The ability of GAP1IP4BP to function as a Rap1 GTPase-activating protein (GAP) requires its Ras GAP-related domain and an arginine finger rather than an asparagine thumb. | Kupzig, S., et al. 2009. Mol Cell Biol. 29: 3929-40. PMID: 19433443
- Modeling the role of G12V and G13V Ras mutations in the Ras-GAP-catalyzed hydrolysis reaction of guanosine triphosphate. | Khrenova, MG., et al. 2014. Biochemistry. 53: 7093-9. PMID: 25339142
- Loss of neurofibromin Ras-GAP activity enhances the formation of cardiac blood islands in murine embryos. | Yzaguirre, AD., et al. 2015. Elife. 4: e07780. PMID: 26460546
- Age-dependent alterations in expression and co-localization of Pax6 and Ras-GAP in brain of aging mice. | Srivastava, K., et al. 2018. J Chem Neuroanat. 92: 25-34. PMID: 29787792
- Identification of the SH3 domain of GAP as an essential sequence for Ras-GAP-mediated signaling. | Duchesne, M., et al. 1993. Science. 259: 525-8. PMID: 7678707
- The GTPase and Rho GAP domains of p190, a tumor suppressor protein that binds the M(r) 120,000 Ras GAP, independently function as anti-Ras tumor suppressors. | Wang, DZ., et al. 1997. Cancer Res. 57: 2478-84. PMID: 9192829
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ras GAP Antibody (D4B4) | sc-64 | 200 µg/ml | $316.00 |