Ras GAP Antibody (B4F8): sc-63

Ras GAP Antibody (B4F8) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Ras GAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Ras GAP (B4F8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ras GAP protein plays a crucial role in regulating the activity of the Ras family of GTPases, which are pivotal in controlling cell proliferation, differentiation, and survival. By facilitating the hydrolysis of GTP to GDP, Ras GAP acts as a negative regulator of Ras signaling, thereby preventing uncontrolled cell growth that can lead to oncogenesis. Mutations in Ras proteins that impair their GTPase activity can result in persistent signaling, contributing to various cancers. The interaction of Ras GAP with p21Ras significantly enhances the GTP hydrolysis rate, underscoring its importance in maintaining cellular homeostasis. Additionally, the product of the neurofibromatosis type 1 gene (NF1) has been identified as a GTPase-activating protein for Ras, further illustrating the intricate regulatory networks involving Ras GAP and its interactions with other proteins that modulate GTPase activity.

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Ras GAP Antibody (B4F8) References:

1. Versatile roles of R-Ras GAP in neurite formation of PC12 cells and embryonic vascular development.  |  Iwashita, S., et al. 2007. J Biol Chem. 282: 3413-7. PMID: 17179160
2. Bcr encodes a GTPase-activating protein for p21rac.  |  Diekmann, D., et al. 1991. Nature. 351: 400-2. PMID: 1903516
3. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21rap1.  |  Rubinfeld, B., et al. 1991. Cell. 65: 1033-42. PMID: 1904317
4. Rap1A antagonizes the ability of Ras and Ras-Gap to inhibit muscarinic K+ channels.  |  Yatani, A., et al. 1991. J Biol Chem. 266: 22222-6. PMID: 1939245
5. The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
6. The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins.  |  Ballester, R., et al. 1990. Cell. 63: 851-9. PMID: 2121371
7. ras GTPase activating protein: signal transmitter and signal terminator.  |  McCormick, F. 1989. Cell. 56: 5-8. PMID: 2535967
8. Cloning of bovine GAP and its interaction with oncogenic ras p21.  |  Vogel, US., et al. 1988. Nature. 335: 90-3. PMID: 2842690
9. Diversity of mechanisms in Ras-GAP catalysis of guanosine triphosphate hydrolysis revealed by molecular modeling.  |  Grigorenko, BL., et al. 2019. Org Biomol Chem. 17: 4879-4891. PMID: 31041977
10. Molecular cloning of two types of GAP complementary DNA from human placenta.  |  Trahey, M., et al. 1988. Science. 242: 1697-700. PMID: 3201259
11. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
12. Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus.  |  Shih, TY., et al. 1980. Nature. 287: 686-91. PMID: 6253810

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ras GAP Antibody (B4F8) has 102 citations in a variety of scientific publications.

Primary antibodies like Anti-Ras GAP Antibody (B4F8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ras GAP Antibody (B4F8) Specifications

Alternate Product Name: RASA1

Product Clone Name: B4F8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Ras GAP

Epitope Species: human

Product Reactivity/Detection: Ras GAP p120

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 102 citations in various scientific publications

Available Conjugates/Forms for Ras GAP Antibody (B4F8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin