Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RAPGEF6 CRISPR/Cas9 KO Plasmid (h): sc-403816
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RAPGEF6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RAPGEF6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RAPGEF6 Antibody (F-8): sc-398642
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAPGEF6 CRISPR/Cas9 KO Plasmid (h) | sc-403816 | 20 µg | $397.00 |
Overview
RAPGEF6 (also known as PDZ-GEF2) encodes a guanine nucleotide exchange factor that activates Rap1 and Rap2 GTPases, linking receptor-initiated signals to small GTPase cycling and downstream effector engagement. Through Rap-dependent control of integrin inside-out signaling, cadherin-mediated adhesion, and cytoskeletal organization, RAPGEF6 contributes to regulation of cell polarity, migration, and junctional stability. Its PDZ and Ras-association domains support scaffolding interactions that coordinate signaling at membranes and adhesion complexes. Dysregulation of RapGEF/Rap signaling has been implicated in altered cell adhesion and motility phenotypes relevant to cancer biology, neurodevelopmental processes, and immune cell trafficking.
RAPGEF6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAPGEF6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAPGEF6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAPGEF6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RAPGEF6 protein expression.
This CRISPR knockout system enables efficient generation of RAPGEF6-deficient cell models for investigation of RAPGEF6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAPGEF6 exon(s) critical for RAPGEF6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAPGEF6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RAPGEF6 CRISPR/Cas9 KO Plasmid (h) and RAPGEF6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAPGEF6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RAPGEF6 HDR Plasmid (h) and RAPGEF6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAPGEF6 homology arms to support homology-directed repair at defined RAPGEF6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.