RAPGEF6 Antibody (F-8): sc-398642
- RAPGEF6 Antibody (F-8) is a mouse monoclonal IgG2a κ RAPGEF6 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-167 mapping at the N-terminus of RAPGEF6 of human origin
- RAPGEF6 Antibody (F-8) is recommended for detection of RAPGEF6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RAPGEF6 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RAPGEF6 Antibody (F-8) for WB applications. These reagents are now offered in bundles with RAPGEF6 Antibody (F-8) (see ordering information below).
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RAPGEF6 Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects RAPGEF6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RAPGEF6 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RAPGEF6, also known as Rap guanine nucleotide exchange factor 6 or PDZGEF2, plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) that activates small GTPases Rap 1 and Rap 2. This activation is vital for various cellular processes, including cell adhesion, proliferation, and differentiation, making RAPGEF6 an important player in pathway regulation. RAPGEF6 is primarily localized in the cytoplasm and translocates to the plasma membrane upon ligand binding, which is essential for signal transduction function. RAPGEF6 contains several functional domains, including an N-terminal Ras-GEF domain, a cyclic nucleotide monophosphate-binding domain, a PDZ domain, a Ras-associating domain, and a Ras exchanger motif, which collectively facilitate interactions with other proteins and regulatory functions. RAPGEF6 interacts with M-Ras through its RA domain, further underscoring significance in cellular signaling networks. Alternative splicing of RAPGEF6 results in two isoforms, PDZ-GEF2A and PDZ-GEF2B, which may have distinct regulatory roles in different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RAPGEF6 Antibody (F-8) References:
- Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras. | Gao, X., et al. 2001. J Biol Chem. 276: 42219-25. PMID: 11524421
- Solution structure of the PDZ2 domain from cytosolic human phosphatase hPTP1E complexed with a peptide reveals contribution of the beta2-beta3 loop to PDZ domain-ligand interactions. | Kozlov, G., et al. 2002. J Mol Biol. 320: 813-20. PMID: 12095257
- Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2. | Kuiperij, HB., et al. 2003. Biochim Biophys Acta. 1593: 141-9. PMID: 12581858
- ESI-MS and FTIR studies of the interaction between the second PDZ domain of hPTP1E and target peptides. | Papp, R., et al. 2003. Biochem Cell Biol. 81: 71-80. PMID: 12870871
- Natural variation and genetic covariance in adult hippocampal neurogenesis. | Kempermann, G., et al. 2006. Proc Natl Acad Sci U S A. 103: 780-5. PMID: 16407118
- Haplotypes spanning SPEC2, PDZ-GEF2 and ACSL6 genes are associated with schizophrenia. | Chen, X., et al. 2006. Hum Mol Genet. 15: 3329-42. PMID: 17030554
- Differential cerebral cortex transcriptomes of baboon neonates consuming moderate and high docosahexaenoic acid formulas. | Kothapalli, KS., et al. 2007. PLoS One. 2: e370. PMID: 17426818
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAPGEF6 Antibody (F-8) | sc-398642 | 200 µg/ml | $322.00 | |||
RAPGEF6 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-530884 | 200 µg Ab; 10 µg BP | $361.00 | |||
RAPGEF6 Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-524435 | 200 µg Ab, 40 µg BP | $361.00 | |||
RAPGEF6 Antibody (F-8): m-IgG2a BP-HRP Bundle | sc-547619 | 200 µg Ab; 10 µg BP | $361.00 | |||
RAPGEF6 Antibody (F-8) AC | sc-398642 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RAPGEF6 Antibody (F-8) HRP | sc-398642 HRP | 200 µg/ml | $322.00 | |||
RAPGEF6 Antibody (F-8) FITC | sc-398642 FITC | 200 µg/ml | $336.00 | |||
RAPGEF6 Antibody (F-8) PE | sc-398642 PE | 200 µg/ml | $349.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 488 | sc-398642 AF488 | 200 µg/ml | $364.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 546 | sc-398642 AF546 | 200 µg/ml | $364.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 594 | sc-398642 AF594 | 200 µg/ml | $364.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 647 | sc-398642 AF647 | 200 µg/ml | $364.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 680 | sc-398642 AF680 | 200 µg/ml | $364.00 | |||
RAPGEF6 Antibody (F-8) Alexa Fluor® 790 | sc-398642 AF790 | 200 µg/ml | $364.00 |