Rap1GAP Antibody (E-12): sc-514387

Rap1GAP Antibody (E-12) is a mouse monoclonal IgG2a antibody that detects Rap1GAP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rap1GAP plays a crucial role in cellular signaling by specifically stimulating the GTP hydrolytic activity of the monomeric G protein Rap1, which is essential for regulating various cellular processes, including cell proliferation and differentiation. Rap1GAP is primarily located in the brain, kidney, and pancreas, where Rap1GAP acts as a signal integrator, coordinating Gz signaling and Rap1 signaling pathways. Notably, Rap1GAP interacts with Gα z, a member of the Gi family of trimeric G proteins, which inhibits the ability of regulators of G protein signaling to promote GTP hydrolysis of the α subunit, thereby modulating downstream signaling events. Additionally, a novel isoform known as Rap1GAPII has been identified, which specifically binds to Gα z and is translocated from the cytosol to the membrane upon stimulation of the Gi-coupled M2 Muscarinic receptor, leading to a decrease in GTP-bound Rap1 and subsequent activation of the ERK/MAPK pathway. anti-Rap1GAP antibody (E-12) is an invaluable tool for researchers studying the intricate mechanisms of G protein signaling and its implications in various physiological and pathological processes.

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Rap1GAP Antibody (E-12) References:

1. Localization of the rap1GAP catalytic domain and sites of phosphorylation by mutational analysis.  |  Rubinfeld, B., et al. 1992. Mol Cell Biol. 12: 4634-42. PMID: 1406653
2. Phosphorylation of rap1GAP in vivo and by cAMP-dependent kinase and the cell cycle p34cdc2 kinase in vitro.  |  Polakis, P., et al. 1992. J Biol Chem. 267: 10780-5. PMID: 1587853
3. Identification of a putative tumor suppressor gene Rap1GAP in pancreatic cancer.  |  Zhang, L., et al. 2006. Cancer Res. 66: 898-906. PMID: 16424023
4. Rap1GAP promotes invasion via induction of matrix metalloproteinase 9 secretion, which is associated with poor survival in low N-stage squamous cell carcinoma.  |  Mitra, RS., et al. 2008. Cancer Res. 68: 3959-69. PMID: 18483282
5. Loss of Rap1GAP in papillary thyroid cancer.  |  Nellore, A., et al. 2009. J Clin Endocrinol Metab. 94: 1026-32. PMID: 19066305
6. Downregulation of Rap1GAP through epigenetic silencing and loss of heterozygosity promotes invasion and progression of thyroid tumors.  |  Zuo, H., et al. 2010. Cancer Res. 70: 1389-97. PMID: 20124489
7. The tumor suppressor gene rap1GAP is silenced by miR-101-mediated EZH2 overexpression in invasive squamous cell carcinoma.  |  Banerjee, R., et al. 2011. Oncogene. 30: 4339-49. PMID: 21532618
8. MiR-3121-3p promotes tumor invasion and metastasis by suppressing Rap1GAP in papillary thyroid cancer in vitro.  |  Xu, M., et al. 2020. Ann Transl Med. 8: 1229. PMID: 33178761
9. Downregulation of Rap1GAP Expression Activates the TGF-β/Smad3 Pathway to Inhibit the Expression of Sodium/Iodine Transporter in Papillary Thyroid Carcinoma Cells.  |  Yan, Z., et al. 2021. Biomed Res Int. 2021: 6840642. PMID: 34840979
10. Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product.  |  Kurachi, H., et al. 1997. J Biol Chem. 272: 28081-8. PMID: 9346962

The preferred Rap1GAP antibody is Rap1GAP Antibody (D-9): sc-166586.