Rap1GAP Antibody (D-9): sc-166586

Rap1GAP Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rap1GAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Rap1GAP antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rap1 GTPase-activating protein (Rap1GAP) plays a crucial role in cellular signaling by specifically stimulating the GTP hydrolytic activity of the monomeric G protein Rap1, which is essential for regulating various cellular processes, including cell adhesion, proliferation, and differentiation. Rap1GAP is primarily located in the cytoplasm and can translocate to the membrane upon stimulation, which is vital for modulating signaling pathways. Notably, Rap1GAP interacts with Gα z, a member of the Gi family of trimeric G proteins, which inhibits the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, thereby influencing downstream signaling events. Rap1GAP expression in the brain, kidney, and pancreas suggests involvement in diverse physiological processes, and the discovery of a novel isoform, Rap1GAPII, which binds specifically to Gα z, highlights the complexity of regulatory mechanisms. Stimulation of the Gi-coupled M2 muscarinic receptor leads to the translocation of Rap1GAPII from the cytosol to the membrane, resulting in decreased levels of GTP-bound Rap1 and subsequent activation of the ERK/MAPK signaling pathway, underscoring Rap1GAP′s importance in integrating G protein signaling and cellular responses.

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Rap1GAP Antibody (D-9) References:

1. Modulation of rap activity by direct interaction of Galpha(o) with Rap1 GTPase-activating protein.  |  Jordan, JD., et al. 1999. J Biol Chem. 274: 21507-10. PMID: 10419452
2. Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with G alpha(i).  |  Mochizuki, N., et al. 1999. Nature. 400: 891-4. PMID: 10476970
3. Functional interaction between Galpha(z) and Rap1GAP suggests a novel form of cellular cross-talk.  |  Meng, J., et al. 1999. J Biol Chem. 274: 36663-9. PMID: 10593970
4. Rap1GAP impairs cell-matrix adhesion in the absence of effects on cell-cell adhesion.  |  Vuchak, LA., et al. 2011. Cell Adh Migr. 5: 323-31. PMID: 21785277
5. Rap1GAP inhibits tumor progression in endometrial cancer.  |  Tamate, M., et al. 2017. Biochem Biophys Res Commun. 485: 476-483. PMID: 28196746
6. Reduced expression of Rap1GAP as a prognostic biomarker for primary gastric cancer patients.  |  Zhao, J., et al. 2018. Cancer Biomark. 22: 375-384. PMID: 29758923
7. Methylation level of Rap1GAP and the clinical significance in MDS.  |  Ding, WJ., et al. 2018. Oncol Lett. 16: 7287-7294. PMID: 30546468
8. Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress.  |  Gao, Y., et al. 2021. Oxid Med Cell Longev. 2021: 7848027. PMID: 33936386
9. Rap1GAP exacerbates myocardial infarction by regulating the AMPK/SIRT1/NF-κB signaling pathway.  |  Shan, T., et al. 2024. Cell Signal. 117: 111080. PMID: 38320624
10. Phosphorylation of Rap1GAP during the cell cycle.  |  Janoueix-Lerosey, I., et al. 1994. Biochem Biophys Res Commun. 202: 967-75. PMID: 8048970
11. Mitogen-inducible SIPA1 is mapped to the conserved syntenic groups of chromosome 19 in mouse and chromosome 11q13.3 centromeric to BCL1 in human.  |  Wada, Y., et al. 1997. Genomics. 39: 66-73. PMID: 9027487
12. Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product.  |  Kurachi, H., et al. 1997. J Biol Chem. 272: 28081-8. PMID: 9346962

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rap1GAP Antibody (D-9) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Rap1GAP Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rap1GAP Antibody (D-9) Specifications

Alternate Product Name: Rap1GAP

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 23-40

Epitope Target: Rap1GAP

Epitope Species: human

Product Reactivity/Detection: Rap1GAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Rap1GAP Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin