RAP Antibody (E-7): sc-515625

RAP Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects RAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RAP Antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RAP protein plays a crucial role as a molecular chaperone that assists in the proper folding and trafficking of the low-density lipoprotein receptor-related protein (LRP), which is essential for the receptor′s function in mediating the uptake of various ligands, including alpha-2-macroglobulin and apolipoprotein E. By binding to LRP, RAP prevents the receptor from interacting with its ligands, thereby regulating the endocytic process and maintaining cellular homeostasis. RAP′s function is underscored by involvement in the pathophysiology of diseases such as Alzheimer′s disease, where LRP′s impaired function can lead to disrupted lipid metabolism and accumulation of amyloid-beta. With interactions with multiple ligands and expression in critical tissues such as the brain, liver, and lung, RAP is vital for understanding both normal physiological processes and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RAP Antibody (E-7) References:

1. Is the LDL receptor-related protein involved in Alzheimer's disease?  |  Lambert, JC., et al. 1999. Neurogenetics. 2: 109-13. PMID: 10369887
2. Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2.  |  Trommsdorff, M., et al. 1999. Cell. 97: 689-701. PMID: 10380922
3. Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms.  |  Mikhailenko, I., et al. 1999. J Cell Sci. 112 (Pt 19): 3269-81. PMID: 10504332
4. The second and fourth cluster of class A cysteine-rich repeats of the low density lipoprotein receptor-related protein share ligand-binding properties.  |  Neels, JG., et al. 1999. J Biol Chem. 274: 31305-11. PMID: 10531329
5. The endocytic receptor protein LRP also mediates neuronal calcium signaling via N-methyl-D-aspartate receptors.  |  Bacskai, BJ., et al. 2000. Proc Natl Acad Sci U S A. 97: 11551-6. PMID: 11016955
6. Modulation of amyloid beta-protein clearance and Alzheimer's disease susceptibility by the LDL receptor-related protein pathway.  |  Kang, DE., et al. 2000. J Clin Invest. 106: 1159-66. PMID: 11067868
7. Role of rap in the biogenesis of lipoprotein receptors.  |  Bu, G. and Marzolo, MP. 2000. Trends Cardiovasc Med. 10: 148-55. PMID: 11239794
8. Three complement-type repeats of the low-density lipoprotein receptor-related protein define a common binding site for RAP, PAI-1, and lactoferrin.  |  Vash, B., et al. 1998. Blood. 92: 3277-85. PMID: 9787164

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RAP Antibody (E-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RAP Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RAP Antibody (E-7) Specifications

Alternate Product Name: RAP

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 230-357

Epitope Target: RAP

Epitope Species: human

Product Reactivity/Detection: RAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RAP Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin