Rap 1A CRISPR/Cas9 KO Plasmid (h): sc-400572
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rap 1A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rap 1A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rap 1A Antibody (C-10): sc-373968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rap 1A CRISPR/Cas9 KO Plasmid (h) | sc-400572 | 20 µg | $397.00 |
Overview
RAP1A encodes Rap1A, a small Ras-family GTPase that cycles between GDP- and GTP-bound states to control inside-out integrin activation, cell adhesion, and cytoskeletal remodeling. Through effectors such as RIAM, RapL, and MAPK signaling intermediates, Rap1A coordinates focal adhesion dynamics, cell polarity, and membrane trafficking that influence migration and barrier function. Rap1A activity integrates inputs from GPCR and receptor tyrosine kinase pathways and contributes to regulation of endothelial junctions and leukocyte adhesion. Dysregulated RAP1A signaling has been linked to altered proliferative and invasive behavior in cancer models and to vascular and inflammatory phenotypes where adhesion-dependent signaling is perturbed.
Rap 1A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAP1A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAP1A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAP1A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rap 1A protein expression.
This CRISPR knockout system enables efficient generation of RAP1A-deficient cell models for investigation of Rap 1A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAP1A exon(s) critical for Rap 1A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAP1A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rap 1A CRISPR/Cas9 KO Plasmid (h) and Rap 1A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAP1A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rap 1A HDR Plasmid (h) and Rap 1A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAP1A homology arms to support homology-directed repair at defined RAP1A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.