RANTES Antibody (F-11) recommended for detection of RANTES of human origin by WB, IP, IF and ELISA

RANTES Antibody (F-11): sc-514019

RANTES Antibody (F-11) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-RANTES Antibody (F-11) is a mouse monoclonal IgG1 (kappa light chain) RANTES antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 24-91 at the C-terminus of RANTES of human origin
    • RANTES Antibody (F-11) is recommended for detection of RANTES of human origin by WB, IP, IF and ELISA
    • Anti-RANTES Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RANTES (F-11): sc-514019.

I ran 20ug of cell lysates from Beas2b and probed with this antibody in the ratio 1:1000 but I got a band only above 25kDa instead of 8kDa. But in your data sheet the band shows 6kDa.Will this antibody detects any precursor? What would u suggest me to do?

Asked by: karti
Thank you for your question. We are not aware of any form of RANTES that would be expected to be observed at a molecular weight above 25 kDa. We would recommend increasing both the amount of protein you are loading, as well as the concentration of primary antibody. For the data shown on our website, we load 50 µg of human platelet extract, and the primary antibody was used at a dilution of 1:200. A link to our recommended Western blotting protocol with this antibody is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you still do not observe the expected size of RANTES, please contact our Technical Service Department and we can assist you with troubleshooting the antibody.
Answered by: Technical Support
Date published: 2017-03-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RANTES (F-11): sc-514019?

Asked by: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great for western blot Work well in WB at 1:100, WPMY-1 cells. Strip of rantese is exact.
Date published: 2017-07-14
Rated 1 out of 5 by from Not good antibody as expected I received this sample antibody from santacruz, but it did not work for me. I used 1: 1000 and 1:500 dilution of antibody and checked them on Beas2b and NHBE cell lysate. But I couldn't see a band less than 10kDa but Im getting a single nice band around 25kDa.
Date published: 2017-03-20
Rated 5 out of 5 by from perfect Strip clear, background clean,WB data of rantese is exact.
Date published: 2016-11-03
Rated 5 out of 5 by from Great Western blot data of RANTES expression Great Western blot data of RANTES expression in human platelet extract. -SCBT QC
Date published: 2013-06-23
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