Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ran BP-1 CRISPR/Cas9 KO Plasmid (m): sc-422595
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ran BP-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ran BP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ran BP-1 Antibody (E-9): sc-514854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ran BP-1 CRISPR/Cas9 KO Plasmid (m) | sc-422595 | 20 µg | $397.00 |
Overview
Ranbp1 encodes Ran-binding protein 1 (Ran BP-1), a conserved regulator of the Ran GTPase cycle that coordinates nucleocytoplasmic transport by modulating RanGTP/RanGDP dynamics. Through its interaction with Ran and RCC1/RanGAP-dependent processes, Ran BP-1 contributes to nuclear import/export, spindle assembly, and mitotic progression, linking transport control to cell-cycle fidelity. Perturbation of the Ran pathway can disrupt genome stability and proliferation, making Ranbp1 function relevant to studies of developmental defects and oncogenic transformation. In mouse systems, Ran BP-1 is frequently examined in the context of nuclear transport-dependent signaling and cell division phenotypes.
Ran BP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ranbp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ranbp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ranbp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ran BP-1 protein expression.
This CRISPR knockout system enables efficient generation of Ranbp1-deficient cell models for investigation of Ran BP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ranbp1 exon(s) critical for Ran BP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ranbp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ran BP-1 CRISPR/Cas9 KO Plasmid (m) and Ran BP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ranbp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ran BP-1 HDR Plasmid (m) and Ran BP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ranbp1 homology arms to support homology-directed repair at defined Ranbp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.