RAGE Antibody (A-9): sc-365154

RAGE Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize the Receptor for Advanced Glycation End Products (RAGE) by targeting a precise epitope mapped between amino acids 23-43 at the N-terminus of human RAGE. RAGE is a crucial type I membrane protein expressed on the surface of endothelial cells, mononuclear phagocytes, and vascular smooth muscle cells, where RAGE functions as a key receptor for advanced glycation end products (AGEs), S100 proteins, and high-mobility group box 1 (HMGB1). The binding of these ligands to RAGE activates several intracellular signaling pathways, including the NF-κB and MAP kinase pathways, which are essential for regulating inflammatory responses, oxidative stress, and cellular proliferation. This functional role of RAGE is significant as RAGE is implicated in the pathogenesis of numerous chronic diseases such as diabetes, atherosclerosis, Alzheimer′s disease, and other inflammatory and vascular disorders. By mediating these processes, RAGE contributes to the progression of vascular dysfunction and amyloidosis, making RAGE a vital target for therapeutic intervention. Anti-RAGE antibody (A-9) is optimized for use in a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), demonstrating robust reactivity with RAGE from mouse, rat, and human origins. The high affinity and specificity of RAGE monoclonal antibody (A-9) ensure reliable and consistent detection of RAGE, facilitating advanced research into the molecular mechanisms by which RAGE contributes to disease progression and supporting the development of targeted therapies aimed at mitigating RAGE-mediated pathological processes. By enabling precise measurement and localization of RAGE, RAGE (A-9) antibody serves as an indispensable tool for researchers seeking to elucidate the intricate roles of RAGE in various disease states and to explore potential strategies for therapeutic intervention.

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RAGE Antibody (A-9) References:

1. Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins.  |  Neeper, M., et al. 1992. J Biol Chem. 267: 14998-5004. PMID: 1378843
2. Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptors/binding proteins.  |  Yan, SD., et al. 1994. J Biol Chem. 269: 9889-97. PMID: 8144582
3. RAGE: a novel cellular receptor for advanced glycation end products.  |  Schmidt, AM., et al. 1996. Diabetes. 45 Suppl 3: S77-80. PMID: 8674899
4. The receptor for advanced glycation end products (RAGE) is a central mediator of the interaction of AGE-beta2microglobulin with human mononuclear phagocytes via an oxidant-sensitive pathway. Implications for the pathogenesis of dialysis-related amyloidosis.  |  Miyata, T., et al. 1996. J Clin Invest. 98: 1088-94. PMID: 8787669
5. Advanced glycation end products, oxidant stress and vascular lesions.  |  Chappey, O., et al. 1997. Eur J Clin Invest. 27: 97-108. PMID: 9061302
6. Characterization and functional analysis of the promoter of RAGE, the receptor for advanced glycation end products.  |  Li, J. and Schmidt, AM. 1997. J Biol Chem. 272: 16498-506. PMID: 9195959
7. Activation of the receptor for advanced glycation end products triggers a p21(ras)-dependent mitogen-activated protein kinase pathway regulated by oxidant stress.  |  Lander, HM., et al. 1997. J Biol Chem. 272: 17810-4. PMID: 9211935

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RAGE Antibody (A-9) has 128 citations in a variety of scientific publications.

Primary antibodies like Anti-RAGE Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RAGE Antibody (A-9) Specifications

Alternate Product Name: RAGE

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 23-43

Epitope Target: RAGE

Epitope Species: human

Product Reactivity/Detection: RAGE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 128 citations in various scientific publications

Available Conjugates/Forms for RAGE Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin