Rag A Antibody (D-1): sc-518209

Rag A Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rag A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rag A Antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Rag A, also known as Rraga or FIP-1, is a 313 amino acid protein located in the cytoplasm and nucleus, belonging to the GTR/RAG GTP-binding protein family. Rag A localization plays a significant role in regulating cellular processes, particularly in transporting proteins from the endoplasmic reticulum to the Golgi apparatus and secretory vesicles, which is essential for maintaining cellular homeostasis and function. Rag A is involved in the RCC1/Ran-GTPase pathway and may directly influence tumor necrosis factor alpha signaling, which is important for cell death induction. Rag A exhibits guanine nucleotide-binding activity but has undetectable intrinsic GTPase activity, highlighting unique functional characteristics within the cellular signaling landscape.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rag A Antibody (D-1) References:

1. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B.  |  Sekiguchi, T., et al. 2001. J Biol Chem. 276: 7246-57. PMID: 11073942
2. A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D.  |  Sekiguchi, T., et al. 2004. J Biol Chem. 279: 8343-50. PMID: 14660641
3. First case of an AIDS patient with systemic mast cell disease associated with FIP1-positive eosinophilia treated with imatinib mesylate therapy.  |  Merante, S., et al. 2006. J Clin Oncol. 24: e6-7. PMID: 16446324
4. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1.  |  Sancak, Y., et al. 2008. Science. 320: 1496-501. PMID: 18497260
5. Cloning of a novel family of mammalian GTP-binding proteins (RagA, RagBs, RagB1) with remote similarity to the Ras-related GTPases.  |  Schürmann, A., et al. 1995. J Biol Chem. 270: 28982-8. PMID: 7499430
6. Mapping of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis.  |  Huber, LA., et al. 1994. Proc Natl Acad Sci U S A. 91: 7874-8. PMID: 8058727
7. RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway.  |  Hirose, E., et al. 1998. J Cell Sci. 111 (Pt 1): 11-21. PMID: 9394008

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rag A Antibody (D-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Rag A Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rag A Antibody (D-1) Specifications

Alternate Product Name: Rag A

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 287-313

Epitope Target: Rag A

Epitope Species: human

Product Reactivity/Detection: Rag A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Rag A Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin