Rad54B Antibody (19-K2): sc-101234

Rad54B Antibody (19-K2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad54B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Rad54B antibody (19-K2) is available as a non-conjugated format. Rad54B (19-K2) antibody recognizes a protein crucial in the DNA double-strand break repair (DSBR) pathway, essential for maintaining genomic stability and preventing cell death, mutations, or neoplastic transformations. Rad54B monoclonal antibody (19-K2) targets a member of the RAD52 family, which includes Rad50, Rad51, and MRE11, all working together to facilitate homologous recombination and repair damaged DNA. Rad54B (19-K2) antibody detects a protein that exhibits ATPase activity with double-stranded DNA, vital for promoting homologous DNA pairing and stimulating recombination processes. Rad54B (19-K2) monoclonal antibody recognizes a protein predominantly expressed in testis and spleen, where mutations in RAD54B have been implicated in various cancers, including primary lymphoma and colon cancer. Anti-Rad54B antibody (19-K2) identifies a protein that interacts with Rad51, critical for DNA damage repair and influenced by BRCA1 and BRCA2, highlighting their interconnected roles in cellular response to DNA damage.

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Rad54B Antibody (19-K2) References:

1. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
2. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
3. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
4. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
5. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
6. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
7. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.  |  Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
8. Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein.  |  Sarai, N., et al. 2006. Nucleic Acids Res. 34: 4429-37. PMID: 16945962
9. The enigmatic oncogene and tumor suppressor-like properties of RAD54B: Insights into genome instability and cancer.  |  McAndrew, EN. and McManus, KJ. 2017. Genes Chromosomes Cancer. 56: 513-523. PMID: 28295846
10. RAD54B mutations enhance the sensitivity of ovarian cancer cells to poly(ADP-ribose) polymerase (PARP) inhibitors.  |  Liu, P., et al. 2022. J Biol Chem. 298: 102354. PMID: 35952757
11. RAD54B inhibits vascular endothelial senescence via suppression of CHK1/p53/p21 pathway.  |  Mai, Y., et al. 2024. Can J Physiol Pharmacol. 102: 137-149. PMID: 37748205
12. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad54B Antibody (19-K2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad54B Antibody (19-K2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad54B Antibody (19-K2) Specifications

Alternate Product Name: Rad54B

Product Clone Name: 19-K2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 801-910

Epitope Target: Rad54B

Epitope Species: human

Product Reactivity/Detection: Rad54B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Rad54B Antibody (19-K2): N/A