Rad54 Antibody (4E3/1): sc-53433
- Rad54 Antibody (4E3/1) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against full length human Rad54
- recommended for detection of Rad54 of human origin by WB, IP and ELISA; also reactive with additional species, including and canine, ovine, rabbit, Guinea pig and monkey
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Rad54 Antibody (4E3/1) for WB applications. This reagent is now offered in a bundle with Rad54 Antibody (4E3/1) (see ordering information below).
Rad54 Antibody (4E3/1) is a mouse monoclonal IgG1 antibody that detects Rad54 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Rad54 is a crucial member of the DEAD-like helicase superfamily, playing a significant role in DNA repair processes, particularly in homologous recombination. Rad54 is primarily located in the nucleus, where anti-Rad54 antibody (4E3/1) can detect binding to double-stranded DNA and induced topological changes that facilitate homologous DNA pairing, thereby stimulating DNA recombination. Rad54′s nuclear localization ensures ready availability to interact with other key players in the DNA repair machinery, such as Rad51 and MRE11, during critical moments of DNA damage response. Mutations in the RAD54 gene have been linked to various cancers, including primary lymphoma and colon cancer, highlighting Rad54′s essential function in maintaining genomic stability. Through interactions with other proteins involved in DNA repair, Rad54 (4E3/1) antibody serves as an invaluable tool for researchers studying DNA damage and repair mechanisms.
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Rad54 Antibody (4E3/1) References:
- Rad54, the motor of homologous recombination. | Mazin, AV., et al. 2010. DNA Repair (Amst). 9: 286-302. PMID: 20089461
- Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination. | Ceballos, SJ. and Heyer, WD. 2011. Biochim Biophys Acta. 1809: 509-23. PMID: 21704205
- Rad54 and Mus81 cooperation promotes DNA damage repair and restrains chromosome missegregation. | Ghamrasni, SE., et al. 2016. Oncogene. 35: 4836-45. PMID: 26876210
- Nek1 Regulates Rad54 to Orchestrate Homologous Recombination and Replication Fork Stability. | Spies, J., et al. 2016. Mol Cell. 62: 903-917. PMID: 27264870
- Rad54 Phosphorylation Promotes Homologous Recombination by Balancing Rad54 Mobility and DNA Binding. | Lengert, N., et al. 2019. Biophys J. 116: 1406-1419. PMID: 30961891
- Rdh54/Tid1 inhibits Rad51-Rad54-mediated D-loop formation and limits D-loop length. | Shah, SS., et al. 2020. Elife. 9: PMID: 33185188
- Discrete roles for Rad54 and Rdh54 during homologous recombination. | Crickard, JB. 2021. Curr Opin Genet Dev. 71: 48-54. PMID: 34293661
- Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments. | Meir, A., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35042797
- TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination Repair. | Ghosh, I., et al. 2023. Nucleic Acids Res. 51: 8643-8662. PMID: 37439356
- Small molecules that disrupt RAD54-BLM interaction hamper tumor proliferation in colon cancer chemoresistance models. | Kaur, E., et al. 2024. J Clin Invest. 134: PMID: 38421735
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad54 Antibody (4E3/1) | sc-53433 | 200 µg/ml | $322.00 | |||
Rad54 Antibody (4E3/1): m-IgG Fc BP-HRP Bundle | sc-538990 | 200 µg Ab; 10 µg BP | $361.00 |