Rad54 Antibody (F-11): sc-374598

Rad54 Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad54 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rad54 antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad54 plays a crucial role in DNA double-strand break repair, a vital process that prevents cell death, mutations, and potential neoplastic transformations. Specifically, Rad54 is part of the DEAD-like helicase superfamily and binds to double-stranded DNA, inducing topological changes that facilitate homologous DNA pairing and stimulate DNA recombination. This function is particularly important in maintaining genomic stability, as improper repair of DNA breaks can lead to severe cellular consequences, including cancer. Rad54 interacts with other key proteins involved in DNA repair, such as Rad51, which is essential for the homologous recombination repair pathway. The interplay between Rad54 and Rad51 is critical, as Rad51′s interaction with BRCA1 and BRCA2 influences cellular responses to DNA damage, and mutations in these pathways can lead to genomic instability and tumorigenesis. Rad54 (F-11) antibody serves as a valuable tool for researchers studying the mechanisms of DNA repair and the implications of these processes in cancer biology.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad54 Antibody (F-11) References:

1. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
2. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
3. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
4. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
5. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
6. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
7. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.  |  Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
8. Isolation and characterization of the RAD54 gene from Arabidopsis thaliana.  |  Osakabe, K., et al. 2006. Plant J. 48: 827-42. PMID: 17227544
9. Human Rad54 protein stimulates human Mus81-Eme1 endonuclease.  |  Mazina, OM. and Mazin, AV. 2008. Proc Natl Acad Sci U S A. 105: 18249-54. PMID: 19017809
10. RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.  |  Mason, JM., et al. 2015. Nucleic Acids Res. 43: 3180-96. PMID: 25765654
11. The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair.  |  Zhou, Q., et al. 2020. Nat Commun. 11: 2639. PMID: 32457312
12. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad54 Antibody (F-11) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad54 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad54 Antibody (F-11) Specifications

Alternate Product Name: RAD54L

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 11-47

Epitope Target: Rad54

Epitope Species: human

Product Reactivity/Detection: Rad54

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for Rad54 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin