Rad52 Antibody (F-7): sc-365341

Rad52 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad52 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Rad52 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad52 protein plays a crucial role in DNA double-strand break repair, a vital process for maintaining genomic stability and preventing cell death, mutations, or neoplastic transformation. Specifically, Rad52 forms a heptameric ring structure that binds to single-stranded DNA ends, facilitating the annealing of complementary strands, which is essential for effective DNA repair. This structural feature allows Rad52 to efficiently mediate the repair process, particularly in conjunction with other proteins in the Rad52 family, such as Rad51, which interacts with BRCA1 and BRCA2 to influence cellular responses to DNA damage. The interplay between these proteins is critical, as inactivation of BRCA2 can lead to genomic instability and contribute to tumorigenesis. Rad52 monoclonal antibody (F-7) is an invaluable tool for researchers studying DNA repair mechanisms and their implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad52 Antibody (F-7) References:

1. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
2. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
3. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
4. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
5. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
6. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
7. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad52 Antibody (F-7) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad52 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad52 Antibody (F-7) Specifications

Alternate Product Name: RAD52

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 119-418

Epitope Target: Rad52

Epitope Species: human

Product Reactivity/Detection: Rad52

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for Rad52 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin