Rad51D Antibody (C-1): sc-398819

Rad51D Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize the C-terminal epitope encompassing amino acids 233-328 of the human Rad51D protein, ensuring precise detection across multiple applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) in mouse, rat, and human samples. Rad51D, also called TRAD, R51H3, Rad51l3, and Trad-d5, is a critical member of the Rad52 family of proteins, playing an essential role in the homologous recombination repair (HRR) pathway that is responsible for repairing DNA double-strand breaks and maintaining genomic stability. Structurally, Rad51D forms part of the BCDX2 complex alongside Rad51B, Rad51C, and XRCC2, which is crucial for the detection and repair of DNA damage by facilitating the formation and stabilization of RAD51 nucleoprotein filaments necessary for effective homologous recombination. Post-translational modifications of Rad51D, such as phosphorylation and ubiquitination, are vital for regulating activity, interactions, and cellular localization, thereby ensuring efficient participation in DNA repair processes. These modifications influence Rad51D′s ability to interact with other key proteins, including BRCA1 and BRCA2, which are integral to the DNA damage response and repair mechanisms. The precise regulation of Rad51D through these post-translational modifications is essential for preventing genomic instability, mutations, and the potential development of cancer. Additionally, Rad51D interacts with other Rad52 family members like Rad52 and Rad54, enhancing the coordination and efficiency of the DNA repair machinery. Anti-Rad51D antibody (C-1) exhibits high specificity with no cross-reactivity against other members of the Rad51 family, providing reliable and accurate results for researchers investigating DNA repair pathways, cancer biology, and genomic integrity. Available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, Rad51D (C-1) antibody offers versatile solutions to meet diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad51D Antibody (C-1) References:

1. Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.  |  French, CA., et al. 2002. J Biol Chem. 277: 19322-30. PMID: 11912211
2. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
3. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
4. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
5. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
6. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
7. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
8. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.  |  Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
9. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad51D Antibody (C-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad51D Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad51D Antibody (C-1) Specifications

Alternate Product Name: Rad51D

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 233-328

Epitope Target: Rad51D

Epitope Species: human

Product Reactivity/Detection: Rad51D

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Rad51D Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin