Rad51C Antibody (2H11): sc-56214
- Rad51C Antibody (2H11) is a mouse monoclonal IgG1 κ Rad51C antibody, cited in 10 publications, provided at 200 µg/ml
- raised against Rad51C of human origin
- Rad51C Antibody (2H11) is recommended for detection of Rad51C of mouse, rat and human origin by WB and IP; non cross-reactive with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3
- Anti-Rad51C Antibody (2H11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rad51C Antibody (2H11). This work is in progress.
Rad51C Antibody (2H11) is a mouse monoclonal IgG1 kappa light chain antibody developed against the human Rad51C protein. Rad51C, also called R51H3 and Rad51l2a, is a pivotal component of the homologous recombination pathway responsible for repairing DNA double-strand breaks within the nucleus. The nuclear localization of Rad51C is crucial for maintaining genomic stability, preventing mutations, and thwarting neoplastic transformations, thereby underscoring its importance in cellular defense against DNA damage. Rad51C monoclonal antibody (2H11) reliably detects Rad51C proteins from mouse, rat, and human origins through western blotting (WB) and immunoprecipitation (IP), while demonstrating no cross-reactivity with other members of the Rad51 family such as Rad51B, Rad51D, Rad51, XRCC2, or XRCC3. Available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, anti-Rad51C antibody (2H11) offers versatile applications for diverse research needs. Rad51C (2H11) antibody facilitates the investigation of DNA repair mechanisms, cell cycle regulation, and the molecular foundations of genomic integrity in proliferating cells, making this reagent an invaluable tool for researchers focused on cancer biology, genetic stability, and cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad51C Antibody (2H11) References:
- Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts. | Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
- Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre. | Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
- In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination. | Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
- In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair. | Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
- The human Rap1 protein complex and modulation of telomere length. | O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
- DNA annealing mediated by Rad52 and Rad59 proteins. | Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
- Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. | Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
- Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae. | Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad51C Antibody (2H11) | sc-56214 | 200 µg/ml | $316.00 | |||
Rad51C Antibody (2H11) AC | sc-56214 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rad51C Antibody (2H11) HRP | sc-56214 HRP | 200 µg/ml | $316.00 | |||
Rad51C Antibody (2H11) FITC | sc-56214 FITC | 200 µg/ml | $330.00 | |||
Rad51C Antibody (2H11) PE | sc-56214 PE | 200 µg/ml | $343.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 488 | sc-56214 AF488 | 200 µg/ml | $357.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 546 | sc-56214 AF546 | 200 µg/ml | $357.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 594 | sc-56214 AF594 | 200 µg/ml | $357.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 647 | sc-56214 AF647 | 200 µg/ml | $357.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 680 | sc-56214 AF680 | 200 µg/ml | $357.00 | |||
Rad51C Antibody (2H11) Alexa Fluor® 790 | sc-56214 AF790 | 200 µg/ml | $357.00 |