Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rad21 CRISPR/Cas9 KO Plasmid (m): sc-422577
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rad21 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rad21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rad21 Antibody (B-2): sc-271601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad21 CRISPR/Cas9 KO Plasmid (m) | sc-422577 | 20 µg | $397.00 |
Overview
Rad21 encodes a core subunit of the cohesin complex that stabilizes sister chromatid cohesion and organizes higher-order chromatin structure. Through cohesin loading and regulated release during the cell cycle, RAD21 supports accurate chromosome segregation, DNA double-strand break repair, and replication fork stability, linking it to genome maintenance pathways. RAD21-mediated chromatin looping also contributes to transcriptional regulation by coordinating enhancer–promoter contacts and interactions with factors such as CTCF. Disruption or dysregulation of cohesin components, including RAD21, is associated with chromosomal instability and cohesin-related developmental phenotypes, making Rad21 a key target for mechanistic studies in mouse models.
Rad21 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rad21 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rad21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rad21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rad21 protein expression.
This CRISPR knockout system enables efficient generation of Rad21-deficient cell models for investigation of Rad21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rad21 exon(s) critical for Rad21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rad21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rad21 CRISPR/Cas9 KO Plasmid (m) and Rad21 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rad21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rad21 HDR Plasmid (m) and Rad21 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rad21 homology arms to support homology-directed repair at defined Rad21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.