Anti-Rad21 Antibody (B-2) recommended for detection of Rad21 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Rad21 Antibody (B-2): sc-271601

Anti-Rad21 Antibody (B-2) is rated 5.0 out of 5 by 8.
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Datasheets

    • Anti-Rad21 Antibody (B-2) is a mouse monoclonal IgM κ Rad21 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 143-352 mapping within an internal region of Rad21 of human origin
    • recommended for detection of Rad21 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad21 (B-2): sc-271601.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Rad21 Antibody (B-2) for WB applications. This reagent is now offered in a bundle with Rad21 Antibody (B-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with Rad21 (B-2): sc-271601 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good It works well in WB from 293T whole cell lysate using either m-IgGκ BP-HRP: sc-516102 or mouse IgM-HRP.
Date published: 2021-07-02
Rated 5 out of 5 by from WB效果还可以 WB,在肠癌细胞中1:500的稀释比可以孵出来一些条带,但比较弱,不知道这种蛋白是不是需要特殊裂解。
Date published: 2018-12-17
Rated 5 out of 5 by from RAD21 抗体 我一个月之前申请了RAD21抗体,货号为sc271601,很高兴,申请成功并且很快到货,最近提取了蛋白样品做了western,结果挺不错的
Date published: 2018-02-01
Rated 5 out of 5 by from Good! Good for WB in MCF7. for wetern blot this one works beautifully.
Date published: 2017-11-30
Rated 5 out of 5 by from Great The antibody can be used for IF staining. I think it is pretty good.
Date published: 2017-07-25
Rated 5 out of 5 by from Worked well with mouse spleen extracts Worked well with mouse spleen extracts
Date published: 2015-10-01
Rated 5 out of 5 by from Strong band of interest recognized in WB Strong band of interest recognized in WB using HeLa and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-06-09
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