Rad17 Antibody (H-3): sc-17761

Rad17 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad17 protein of human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Rad17 monoclonal antibody (H-3) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Rad17 protein plays a crucial role in DNA damage response by facilitating activation of cell cycle checkpoints, which are essential for maintaining genomic integrity. Rad17 (H-3) antibody is involved in formation of 9-1-1 complex, which is critical for recruitment of repair factors to sites of DNA damage. This recruitment allows for proper repair of damaged DNA before cell proceeds to replicate, thereby preventing propagation of mutations that could lead to cancer or other genetic disorders. Rad17 interaction with other checkpoint proteins, such as Rad9 and Mec3, underscores importance in orchestrating coordinated response to DNA damage, ensuring cells can effectively manage and repair genomic lesions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad17 Antibody (H-3) References:

1. Rad17, the clamp loader that loads more than clamps.  |  Paull, TT. and Lee, JH. 2014. EMBO J. 33: 783-5. PMID: 24619000
2. Human Rad17 C-terminal tail is phosphorylated by concerted action of CK1δ/ε and CK2 to promote interaction with the 9-1-1 complex.  |  Fukumoto, Y., et al. 2019. Biochem Biophys Res Commun. 517: 310-316. PMID: 31353086
3. Nuclear translocation promotes proteasomal degradation of human Rad17 protein through the N-terminal destruction boxes.  |  Fukumoto, Y., et al. 2021. J Biol Chem. 297: 100831. PMID: 34174284
4. The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase.  |  Li, R., et al. 1993. Nature. 366: 82-4. PMID: 8232541
5. DUN1 encodes a protein kinase that controls the DNA damage response in yeast.  |  Zhou, Z. and Elledge, SJ. 1993. Cell. 75: 1119-27. PMID: 8261511
6. Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways.  |  Sanchez, Y., et al. 1996. Science. 271: 357-60. PMID: 8553072
7. Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).  |  Yamamoto, A., et al. 1996. J Cell Biol. 133: 99-110. PMID: 8601617
8. Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae.  |  Siede, W., et al. 1996. Nucleic Acids Res. 24: 1669-75. PMID: 8649984
9. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase.  |  Longhese, MP., et al. 1996. Mol Cell Biol. 16: 3235-44. PMID: 8668138
10. A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription.  |  Aboussekhra, A., et al. 1996. EMBO J. 15: 3912-22. PMID: 8670896
11. A meiotic recombination checkpoint controlled by mitotic checkpoint genes.  |  Lydall, D., et al. 1996. Nature. 383: 840-3. PMID: 8893012
12. RAD9 and DNA polymerase epsilon form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae.  |  Navas, TA., et al. 1996. Genes Dev. 10: 2632-43. PMID: 8895664

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad17 Antibody (H-3) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad17 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad17 Antibody (H-3) Specifications

Alternate Product Name: Rad17

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 371-670

Epitope Target: Rad17

Epitope Species: human

Product Reactivity/Detection: Rad17

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Rad17 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin