RACK7 CRISPR/Cas9 KO Plasmid (h): sc-411774
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RACK7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RACK7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RACK7 Antibody (RF-9): sc-100824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RACK7 CRISPR/Cas9 KO Plasmid (h) | sc-411774 | 20 µg | $397.00 |
Overview
ZMYND8 (RACK7) encodes a chromatin reader that binds acetylated and methylated histones via its PHD–bromodomain–PWWP module to coordinate transcriptional regulation. RACK7 participates in chromatin remodeling and DNA damage response programs, integrating signals from histone modifications to modulate enhancer activity and RNA polymerase II–dependent transcription. It has been linked to regulation of epithelial–mesenchymal transition, hypoxia-associated transcriptional networks, and control of inflammatory gene expression through interactions with corepressor and remodeling complexes. Dysregulation of ZMYND8-associated chromatin pathways has been reported in cancer and other disorders characterized by altered genome stability and transcriptional control.
RACK7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZMYND8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZMYND8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZMYND8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RACK7 protein expression.
This CRISPR knockout system enables efficient generation of ZMYND8-deficient cell models for investigation of RACK7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZMYND8 exon(s) critical for RACK7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZMYND8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RACK7 CRISPR/Cas9 KO Plasmid (h) and RACK7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZMYND8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RACK7 HDR Plasmid (h) and RACK7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZMYND8 homology arms to support homology-directed repair at defined ZMYND8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.