RACK1 Antibody (B-3): sc-17754
- RACK1 Antibody (B-3) is a mouse monoclonal IgG2a κ RACK1 antibody, cited in 92 publications, provided at 200 µg/ml
- raised against amino acids 131-317 of RACK1 of human origin
- RACK1 Antibody (B-3) is recommended for detection of RACK1 of mouse, rat, human and origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-RACK1 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RACK1 Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with RACK1 Antibody (B-3) (see ordering information below).
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RACK1 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that specifically targets amino acids 131-317 of RACK1, also known as Receptor for Activated C Kinase 1, and GNB2L1. RACK1 is a 317-amino-acid multifunctional scaffold protein resembling the G protein β subunit, playing a crucial role in various cellular processes such as cell growth, differentiation, and signal transduction. RACK1 functions as an anchoring protein for activated Protein Kinase C (PKC), facilitating PKC′s translocation and interaction with substrates, an essential process for regulating signaling pathways that control gene expression, hormone secretion, and membrane functions. By inhibiting Src tyrosine kinases and interacting with numerous signaling molecules, RACK1 modulates pathways important for cell adhesion, migration, and proliferation, making RACK1 significant in studies of cancer and other diseases. Anti-RACK1 antibody (B-3) reacts with RACK1 from mouse, rat, human, and additional species including equine, canine, bovine, porcine, and avian, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). RACK1′s role as a scaffold protein is vital for coordinating multiple signaling pathways, and RACK1′s conservation across diverse species underscores RACK1′s importance in fundamental cellular functions. Notably, deficits in RACK1 may be associated with impaired PKC activation in the aging brain, highlighting RACK1′s relevance in neurological studies. Therefore, RACK1 (B-3) antibody provides a valuable tool for advancing research in critical cellular mechanisms and exploring potential therapeutic targets, aiding in the understanding of diseases where RACK1 is implicated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RACK1 Antibody (B-3) References:
- A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. | Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
- Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. | Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
- Expression and characterization of protein kinase C-delta. | Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
- Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. | Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
- Turnover of inositol phospholipids and signal transduction. | Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
- The role of protein kinase C in cell surface signal transduction and tumour promotion. | Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
- Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. | Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
- Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. | Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RACK1 Antibody (B-3) | sc-17754 | 200 µg/ml | $322.00 | |||
RACK1 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-528297 | 200 µg Ab; 10 µg BP | $361.00 | |||
RACK1 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-520638 | 200 µg Ab, 40 µg BP | $361.00 | |||
RACK1 Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-547062 | 200 µg Ab; 10 µg BP | $361.00 | |||
RACK1 Antibody (B-3) AC | sc-17754 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RACK1 Antibody (B-3) HRP | sc-17754 HRP | 200 µg/ml | $322.00 | |||
RACK1 Antibody (B-3) FITC | sc-17754 FITC | 200 µg/ml | $336.00 | |||
RACK1 Antibody (B-3) PE | sc-17754 PE | 200 µg/ml | $349.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 488 | sc-17754 AF488 | 200 µg/ml | $364.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 546 | sc-17754 AF546 | 200 µg/ml | $364.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 594 | sc-17754 AF594 | 200 µg/ml | $364.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 647 | sc-17754 AF647 | 200 µg/ml | $364.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 680 | sc-17754 AF680 | 200 µg/ml | $364.00 | |||
RACK1 Antibody (B-3) Alexa Fluor® 790 | sc-17754 AF790 | 200 µg/ml | $364.00 |