Anti-RACK1 Antibody (B-3) recommended for detection of RACK1 of mouse, rat, human and origin by WB, IP, IF and IHC(P)

Anti-RACK1 Antibody (B-3): sc-17754

Anti-RACK1 Antibody (B-3) is rated 5.0 out of 5 by 22.
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Datasheets

    • Anti-RACK1 Antibody (B-3) is a mouse monoclonal IgG2a κ RACK1 antibody, cited in 71 publications, provided at 200 µg/ml
    • raised against amino acids 131-317 of RACK1 of human origin
    • RACK1 Antibody (B-3) is recommended for detection of RACK1 of mouse, rat, human and origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-RACK1 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RACK1 (B-3): sc-17754.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RACK1 Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with RACK1 Antibody (B-3) (see ordering information below).
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Does this Rack1 monoclonal Ab work against C. elegans rack-1 like your discontinued rabbit polyclonal Ab?

Asked by: DDCsta
Thank you for your question. This monoclonal antibody was raised against the same antigen used to develop the discontinued rabbit polyclonal antibody, sc-10775: RACK1 (H-187). We do not have any data or citations yet using this monoclonal antibody in C. elegans.
Answered by: Technical Support
Date published: 2019-05-25

I have tried this antibody FITC conjugated against mouse RACK1 in flow cytometry application. It did not appear to have any signal. Do you know if this antibody has been tested against mouse Rack1 in flow? Thanks!

Asked by: JZ2019
This antibody has not yet been tested for Flow cytometry (FCM), so this is not a recommended application at this time. The antibody is recommended for detection of RACK1 by WB, IP and IHC. The antibody is mouse, rat and human reactive.
Answered by: Tech Service
Date published: 2019-03-28

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our RACK1 sc-17754 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 5 out of 5 by from Nice and single band for WB This ab works very well in COS7, Hela, and MDA-MB-231 cells. Detects a single band around 35kDa. Ab incubation: 1:1000 in 5% milk in TBST for 2h at RT.
Date published: 2021-04-30
Rated 5 out of 5 by from great works very well for U2OS cells. Detects a strong single band around 35kDa
Date published: 2019-10-22
Rated 5 out of 5 by from Great! This works very well in WB (5% milk, 1:200). Very specific and strong signals.
Date published: 2018-11-13
Rated 5 out of 5 by from Strong and specific signal This antibody works really well. Using the dilution 1:1000 and 1:5000-1:7000 for the secondary Ab I have got a strong signal at 36 kDa and no background.
Date published: 2018-03-26
Rated 5 out of 5 by from works very well works great for western - samples were human cell in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Good for IF This RACK1 antibody shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from Anti-RACK1 antibody Mouse anti-RACK1 antibody (sc--17754) works great on immunoblotting at 1:1000 in 2.5 % milk powder (and can be reused up to 4 times). It works well on IHC at 1:200 and works great on IP. It recognizes a 35 kDa band in all cell lines tested so far.
Date published: 2017-08-24
Rated 5 out of 5 by from Excellent in WB and IF Our lab bought this antibody for use in WB and we have the conjugated antibody for IF. Both work great with excellent specificity. Very pleased with this monoclonal antibody.
Date published: 2017-08-16
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