rabphilin-3A Antibody (E-2): sc-390915

Rabphilin-3A Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects rabphilin-3A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rabphilin-3A is a crucial protein involved in the regulation of calcium-dependent exocytosis, a process essential for neurotransmitter release and hormone secretion in neuroendocrine cells. Rabphilin-3A features a unique structure characterized by two C2 domains at its carboxy-terminal end and a cysteine-rich region with two zinc finger motifs at its amino-terminal, which allows interaction with calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles. This structural configuration enables rabphilin-3A to bind to Rab3A, a known negative regulator of exocytosis, thereby acting as a positive regulator in this critical cellular process. While rabphilin-3A and Rab3A co-localize on synaptic vesicles and chromaffin granules, these proteins appear to influence exocytosis independently, suggesting that rabphilin-3A may exert effects through interactions with an unidentified factor that recognizes its Rab3 binding domain. Anti-rabphilin-3A antibody (E-2) serves as an invaluable tool for researchers studying the mechanisms of exocytosis and the intricate signaling pathways involved in neurotransmitter release.

The preferred rabphilin-3A antibody is rabphilin-3A Antibody (D-6): sc-393197.