RABIF Antibody (D-12): sc-390759

RABIF Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects RABIF protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RABIF antibody (D-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RABIF protein, also known as MSS4, RASGFR3, or RASGRF3, is a 123 amino acid guanine-nucleotide-releasing protein that plays a crucial role in vesicular transport, which is essential for proper functioning of cellular processes. Specifically, RABIF is involved in exchange of guanosine triphosphate (GTP) for guanosine diphosphate (GDP) in Rab proteins, facilitating their activation and subsequent involvement in intracellular trafficking. This process is vital for transport of newly synthesized proteins from endoplasmic reticulum to Golgi apparatus and ultimately to secretory vesicles, ensuring proteins reach their correct destinations within cells. Additionally, RABIF interacts with a subset of Rab proteins, highlighting its importance in regulation of endocytic pathways and overall maintenance of cellular homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RABIF Antibody (D-12) References:

1. Structure of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones.  |  Thaw, P., et al. 2001. Nat Struct Biol. 8: 701-4. PMID: 11473261
2. Mammalian suppressor of Sec4 modulates the inhibitory effect of Rab15 during early endocytosis.  |  Strick, DJ., et al. 2002. J Biol Chem. 277: 32722-9. PMID: 12105226
3. Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian MSS4-Rab8 GTPase protein complex.  |  Itzen, A., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 113-6. PMID: 16511278
4. Novel real-time PCR assay for rapid prenatal diagnosis of Down syndrome: a prospective study of 563 amniocytes.  |  Zhu, YN., et al. 2009. Clin Biochem. 42: 672-5. PMID: 19232337
5. RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.  |  Gulbranson, DR., et al. 2017. Proc Natl Acad Sci U S A. 114: E8224-E8233. PMID: 28894007
6. Identification of the Novel Tumor Suppressor Role of FOCAD/miR-491-5p to Inhibit Cancer Stemness, Drug Resistance and Metastasis via Regulating RABIF/MMP Signaling in Triple Negative Breast Cancer.  |  Huang, WC., et al. 2021. Cells. 10: PMID: 34685504
7. RABIF promotes hepatocellular carcinoma progression through regulation of mitophagy and glycolysis.  |  Feng, N., et al. 2024. Commun Biol. 7: 1333. PMID: 39414994
8. Cloning, Zn2+ binding, and structural characterization of the guanine nucleotide exchange factor human Mss4.  |  Yu, H. and Schreiber, SL. 1995. Biochemistry. 34: 9103-10. PMID: 7619808
9. Structure of guanine-nucleotide-exchange factor human Mss4 and identification of its Rab-interacting surface.  |  Yu, H. and Schreiber, SL. 1995. Nature. 376: 788-91. PMID: 7651540
10. Specific interactions of Mss4 with members of the Rab GTPase subfamily.  |  Burton, JL., et al. 1994. EMBO J. 13: 5547-58. PMID: 7988552
11. Comparison of kinetic properties between MSS4 and Rab3A GRF GDP/GTP exchange proteins.  |  Miyazaki, A., et al. 1994. FEBS Lett. 350: 333-6. PMID: 8070588
12. Cloning of novel transcripts of the human guanine-nucleotide-exchange factor Mss4: in situ chromosomal mapping and expression in pancreatic cancer.  |  Müller-Pillasch, F., et al. 1997. Genomics. 46: 389-96. PMID: 9441742

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RABIF Antibody (D-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RABIF Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RABIF Antibody (D-12) Specifications

Alternate Product Name: RABIF

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-123

Epitope Target: RABIF

Epitope Species: human

Product Reactivity/Detection: RABIF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RABIF Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin