Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab GDI β CRISPR/Cas9 KO Plasmid (h): sc-404136
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab GDI β CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab GDI β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab GDI β Antibody (D-2): sc-515143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab GDI β CRISPR/Cas9 KO Plasmid (h) | sc-404136 | 20 µg | $397.00 |
Overview
GDI2 encodes Rab GDP dissociation inhibitor beta (Rab GDI β), a cytosolic regulator that binds prenylated Rab GTPases and controls their cycling between membranes and the cytosol. By modulating Rab localization and availability for activation, Rab GDI β helps coordinate vesicle budding, tethering, and fusion steps essential for endocytic trafficking, recycling pathways, and Golgi-to-endosome transport. Perturbation of Rab GDI β function can disrupt membrane trafficking homeostasis, affecting receptor turnover, signaling compartmentalization, and organelle dynamics. Altered expression or regulation of Rab GDI2 has been examined in the context of cellular phenotypes relevant to cancer biology and neurobiology where vesicular transport and proteostasis are critical.
Rab GDI β CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GDI2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GDI2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GDI2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab GDI β protein expression.
This CRISPR knockout system enables efficient generation of GDI2-deficient cell models for investigation of Rab GDI β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GDI2 exon(s) critical for Rab GDI β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GDI2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab GDI β CRISPR/Cas9 KO Plasmid (h) and Rab GDI β CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GDI2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab GDI β HDR Plasmid (h) and Rab GDI β HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GDI2 homology arms to support homology-directed repair at defined GDI2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.