Date published: 2025-10-19

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Rab 5B Antibody (F-9): sc-373725

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Datasheets
  • Rab 5B Antibody (F-9) is a mouse monoclonal IgG1 κ Rab 5B antibody, cited in 4 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 186-219 within the C-terminus of Rab 5B of human origin
  • Rab 5B Antibody (F-9) is recommended for detection of Rab 5B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
  • Anti-Rab 5B Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 5B Antibody (F-9) for WB applications. These reagents are now offered in bundles with Rab 5B Antibody (F-9) (see ordering information below).

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Rab 5B Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rab 5B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rab 5B antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 5B protein plays a crucial role in intracellular membrane trafficking, particularly in regulating endocytosis and protein transport from endoplasmic reticulum to Golgi apparatus. This function maintains cellular homeostasis and ensures proteins reach correct destinations within cells, essential for proper cellular function and signaling. Rab proteins, including Rab 5B, participate in vesicle formation and movement, which are membrane-bound structures transporting materials within cells. Rab protein specificity for distinct membrane transport pathway stages highlights their importance in cellular logistics, and dysfunction can lead to various diseases, including neurodegenerative disorders and cancer. Using Rab 5B (F-9) antibody in research provides insights into vesicular transport mechanisms and Rab protein roles in cellular processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Rab 5B Antibody (F-9) References:

  1. GTP-binding proteins in intracellular transport.  |  Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
  2. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach.  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  3. Cloning of a Rab3 isotype predominantly expressed in adipocytes.  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
  4. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
  5. Rab5b function is essential to acquire heme from hemoglobin endocytosis for survival of Leishmania.  |  Rastogi, R., et al. 2021. Biochim Biophys Acta Mol Cell Res. 1868: 118868. PMID: 33011192
  6. Endosomal Trafficking Bypassed by the RAB5B-CD109 Interplay Promotes Axonogenesis in KRAS-Mutant Pancreatic Cancer.  |  Zhang, D., et al. 2024. Adv Sci (Weinh). 11: e2405092. PMID: 39488792
  7. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
  8. Coatomers and SNAREs in promoting membrane traffic.  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
  9. Friends and family: the role of the Rab GTPases in vesicular traffic.  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
  10. The role of GTP-binding proteins in transport along the exocytic pathway.  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
  11. Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation.  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
  12. Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins.  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rab 5B Antibody (F-9)

sc-373725
200 µg/ml
$316.00

Rab 5B Antibody (F-9): m-IgG Fc BP-HRP Bundle

sc-529544
200 µg Ab; 10 µg BP
$354.00

Rab 5B Antibody (F-9): m-IgGκ BP-HRP Bundle

sc-522471
200 µg Ab, 40 µg BP
$354.00

Rab 5B Antibody (F-9): m-IgG1 BP-HRP Bundle

sc-543570
200 µg Ab; 20 µg BP
$354.00

Rab 5B Antibody (F-9) AC

sc-373725 AC
500 µg/ml, 25% agarose
$416.00

Rab 5B Antibody (F-9) HRP

sc-373725 HRP
200 µg/ml
$316.00

Rab 5B Antibody (F-9) FITC

sc-373725 FITC
200 µg/ml
$330.00

Rab 5B Antibody (F-9) PE

sc-373725 PE
200 µg/ml
$343.00

Rab 5B Antibody (F-9) Alexa Fluor® 488

sc-373725 AF488
200 µg/ml
$357.00

Rab 5B Antibody (F-9) Alexa Fluor® 546

sc-373725 AF546
200 µg/ml
$357.00

Rab 5B Antibody (F-9) Alexa Fluor® 594

sc-373725 AF594
200 µg/ml
$357.00

Rab 5B Antibody (F-9) Alexa Fluor® 647

sc-373725 AF647
200 µg/ml
$357.00

Rab 5B Antibody (F-9) Alexa Fluor® 680

sc-373725 AF680
200 µg/ml
$357.00

Rab 5B Antibody (F-9) Alexa Fluor® 790

sc-373725 AF790
200 µg/ml
$357.00

Rab 5B (F-9) Neutralizing Peptide

sc-373725 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373725 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Rab 5B expression in NIH/3T3 whole cell lysate.
Date published: 2017-02-10
Rated 5 out of 5 by from good.Western blot, 1:1000, loading 20ug, in AsPC-1 & MIA PaCa-2 Cell lines. Got good band.
Date published: 2017-02-06
Rated 5 out of 5 by from very good.We asked free test sample from Santa and got good band for western blot. Then we buy a new one. Good antibody. Loading 10-20ug, 1:1000 dilution, easy to get strong band in HEK293T, AsPC-1, MIA PaCa-2 cell lines.
Date published: 2016-12-10
Rated 5 out of 5 by from Very clear and single band in NIH/3T3 wholeVery clear and single band in NIH/3T3 whole cell lysate by WB. -SCBT QC
Date published: 2014-02-21
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Rab 5B Antibody (F-9) is rated 5.0 out of 5 by 5.
  • y_2025, m_10, d_14, h_6
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