Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 33B CRISPR/Cas9 KO Plasmid (h): sc-404070
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 33B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 33B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 33B Antibody (RR-3): sc-81920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 33B CRISPR/Cas9 KO Plasmid (h) | sc-404070 | 20 µg | $397.00 |
Overview
RAB33B encodes the small GTPase Rab 33B, a member of the Rab family that coordinates membrane trafficking events within the Golgi–ER interface. Rab 33B regulates vesicle formation, cargo sorting, and Golgi homeostasis, and has been implicated in autophagy-linked trafficking steps that influence organelle dynamics. Through these roles it contributes to secretory pathway organization and intracellular transport fidelity, processes frequently perturbed in neurodevelopmental and neurodegenerative phenotypes. Altered RAB33B function has been associated with syndromic skeletal and developmental disorders, supporting its relevance for studying trafficking-dependent cellular homeostasis.
Rab 33B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAB33B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAB33B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAB33B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 33B protein expression.
This CRISPR knockout system enables efficient generation of RAB33B-deficient cell models for investigation of Rab 33B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAB33B exon(s) critical for Rab 33B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAB33B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 33B CRISPR/Cas9 KO Plasmid (h) and Rab 33B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAB33B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 33B HDR Plasmid (h) and Rab 33B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAB33B homology arms to support homology-directed repair at defined RAB33B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.