Rab 3 GAP p130 Antibody (G-3): sc-393540
- Rab 3 GAP p130 Antibody (G-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 901-924 near the C-terminus of Rab 3 GAP p130 of human origin
- Rab 3 GAP p130 Antibody (G-3) is recommended for detection of Rab 3 GAP p130 of human origin by WB, IP, IF and ELISA
- Anti-Rab 3 GAP p130 Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 3 GAP p130 Antibody (G-3) for WB applications. These reagents are now offered in bundles with Rab 3 GAP p130 Antibody (G-3) (see ordering information below).
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Rab 3 GAP p130 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rab 3 GAP p130 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rab 3 GAP p130 (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 3 GAP p130, also known as Rab3 GTPase-activating protein catalytic subunit, plays a crucial role in regulating neurotransmitter and hormone exocytosis by converting active RAB3-GTP to inactive RAB3-GDP. This conversion maintains proper synaptic function and neurodevelopment, as required for normal eye and brain development. Defects in Rab 3 GAP p130 are linked to Warburg micro syndrome 1 (WARBM1), a severe autosomal recessive disorder characterized by significant developmental abnormalities in the eye and central nervous system, as well as microgenitalia. Rab 3 GAP p130 consists of 981 amino acids and exists in two alternatively spliced isoforms, highlighting functional diversity. Rab 3 GAP p130 is conserved across several species, including chimpanzees, dogs, cows, mice, chickens, zebrafish, and fruit flies, and is located on human chromosome 2q21.3. Rab 3 GAP p130′s participation in neurodevelopmental processes such as proliferation, migration, and differentiation prior to synapse formation underscores importance in cellular signaling and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rab 3 GAP p130 Antibody (G-3) References:
- Biochemical characterization of Rab3-GTPase-activating protein reveals a mechanism similar to that of Ras-GAP. | Clabecq, A., et al. 2000. J Biol Chem. 275: 31786-91. PMID: 10859313
- Mutations of the catalytic subunit of RAB3GAP cause Warburg Micro syndrome. | Aligianis, IA., et al. 2005. Nat Genet. 37: 221-3. PMID: 15696165
- Rab3 GTPase-activating protein regulates synaptic transmission and plasticity through the inactivation of Rab3. | Sakane, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 10029-34. PMID: 16782817
- New RAB3GAP1 mutations in patients with Warburg Micro Syndrome from different ethnic backgrounds and a possible founder effect in the Danish. | Morris-Rosendahl, DJ., et al. 2010. Eur J Hum Genet. 18: 1100-6. PMID: 20512159
- Isolation and characterization of a GTPase activating protein specific for the Rab3 subfamily of small G proteins. | Fukui, K., et al. 1997. J Biol Chem. 272: 4655-8. PMID: 9030515
- Localization of the Rab3 small G protein regulators in nerve terminals and their involvement in Ca2+-dependent exocytosis. | Oishi, H., et al. 1998. J Biol Chem. 273: 34580-5. PMID: 9852129
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 3 GAP p130 Antibody (G-3) | sc-393540 | 200 µg/ml | $322.00 | |||
Rab 3 GAP p130 Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-530487 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rab 3 GAP p130 Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-523986 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rab 3 GAP p130 Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-544082 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rab 3 GAP p130 Antibody (G-3) AC | sc-393540 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rab 3 GAP p130 Antibody (G-3) HRP | sc-393540 HRP | 200 µg/ml | $322.00 | |||
Rab 3 GAP p130 Antibody (G-3) FITC | sc-393540 FITC | 200 µg/ml | $336.00 | |||
Rab 3 GAP p130 Antibody (G-3) PE | sc-393540 PE | 200 µg/ml | $349.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 488 | sc-393540 AF488 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 546 | sc-393540 AF546 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 594 | sc-393540 AF594 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 647 | sc-393540 AF647 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 680 | sc-393540 AF680 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 Antibody (G-3) Alexa Fluor® 790 | sc-393540 AF790 | 200 µg/ml | $364.00 | |||
Rab 3 GAP p130 (G-3) Neutralizing Peptide | sc-393540 P | 100 µg/0.5 ml | $69.00 |