Rab 1A CRISPR/Cas9 KO Plasmid (m): sc-422550
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 1A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 1A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 1A Antibody (G-10): sc-377201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 1A CRISPR/Cas9 KO Plasmid (m) | sc-422550 | 20 µg | $397.00 |
Overview
Rab1a encodes Rab 1A, a small GTPase that regulates early secretory trafficking by coordinating ER-to-Golgi and intra-Golgi vesicle transport, including vesicle budding, tethering, and fusion events. Through cycling between GTP- and GDP-bound states, Rab 1A engages effector complexes that influence Golgi organization, protein processing, and cargo delivery, thereby supporting proteostasis and organelle homeostasis. Rab1a-dependent trafficking interfaces with autophagy and lysosomal pathways by controlling membrane supply and compartment maturation. Dysregulation of Rab1a-associated transport has been linked in the literature to cellular stress responses and phenotypes relevant to neurodegeneration and oncogenic signaling contexts, making it a useful node for studying membrane traffic–driven disease mechanisms in mouse models.
Rab 1A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rab1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rab1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rab1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 1A protein expression.
This CRISPR knockout system enables efficient generation of Rab1a-deficient cell models for investigation of Rab 1A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rab1a exon(s) critical for Rab 1A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rab1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 1A CRISPR/Cas9 KO Plasmid (m) and Rab 1A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rab1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 1A HDR Plasmid (m) and Rab 1A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rab1a homology arms to support homology-directed repair at defined Rab1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.