Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 14 CRISPR/Cas9 KO Plasmid (m): sc-427035
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 14 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 14 Antibody (D-5): sc-271401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 14 CRISPR/Cas9 KO Plasmid (m) | sc-427035 | 20 µg | $397.00 |
Overview
Rab14 encodes Rab 14, a small GTPase that regulates vesicular trafficking between endosomes, the Golgi apparatus, and the plasma membrane, coordinating cargo sorting and membrane recycling. Rab14 participates in endocytic recycling and secretory transport processes that shape receptor availability, nutrient transporter localization, and membrane dynamics. Through its roles in endosome maturation and trafficking node organization, Rab14 influences pathways linked to cell polarity, cytokinesis, and metabolic regulation. Dysregulated Rab14-dependent trafficking has been associated with phenotypes relevant to oncogenic signaling, insulin-responsive transport, and pathogen-containing vacuole remodeling in experimental systems.
Rab 14 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rab14 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rab14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rab14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 14 protein expression.
This CRISPR knockout system enables efficient generation of Rab14-deficient cell models for investigation of Rab 14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rab14 exon(s) critical for Rab 14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rab14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 14 CRISPR/Cas9 KO Plasmid (m) and Rab 14 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rab14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 14 HDR Plasmid (m) and Rab 14 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rab14 homology arms to support homology-directed repair at defined Rab14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.