Date published: 2025-10-19

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Rab 14 Antibody (D-5): sc-271401

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Datasheets
  • Rab 14 Antibody (D-5) is a mouse monoclonal IgG1, cited in 11 publications, provided at 200 µg/ml
  • raised against amino acids 161-215 mapping at the C-terminus of Rab 14 of human origin
  • Rab 14 Antibody (D-5) is recommended for detection of Rab 14 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
  • Anti-Rab 14 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 14 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 14 Antibody (D-5) (see ordering information below).

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Rab 14 Antibody (D-5) is a mouse monoclonal IgG1 antibody that detects Rab 14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rab 14 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 14, also known as FBP, is a 215 amino acid protein that plays a crucial role in vesicular trafficking and neurotransmitter release, making Rab 14 essential for cellular communication and transport processes. Rab 14 is lipid-anchored to the cytoplasmic side of the cell membrane and is predominantly expressed in the brain, lung, kidney, spleen, and thymus, highlighting Rab 14′s importance in various physiological functions. As a member of the Rab subfamily of small GTPases, Rab 14 is integral to the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi complex and secretory vesicles, as well as in endocytic pathways, thereby facilitating the movement of carrier vesicles essential for maintaining cellular homeostasis and signaling.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Rab 14 Antibody (D-5) References:

  1. Intracellular membrane trafficking pathways in bone-resorbing osteoclasts revealed by cloning and subcellular localization studies of small GTP-binding rab proteins.  |  Zhao, H., et al. 2002. Biochem Biophys Res Commun. 293: 1060-5. PMID: 12051767
  2. Rab14 is involved in membrane trafficking between the Golgi complex and endosomes.  |  Junutula, JR., et al. 2004. Mol Biol Cell. 15: 2218-29. PMID: 15004230
  3. Large scale screening for novel rab effectors reveals unexpected broad Rab binding specificity.  |  Fukuda, M., et al. 2008. Mol Cell Proteomics. 7: 1031-42. PMID: 18256213
  4. Annexin A2 interactions with Rab14 in alveolar type II cells.  |  Gou, D., et al. 2008. J Biol Chem. 283: 13156-64. PMID: 18332131
  5. Rab14 regulates apical targeting in polarized epithelial cells.  |  Kitt, KN., et al. 2008. Traffic. 9: 1218-31. PMID: 18429929
  6. Membrane traffic and polarization of lipid domains during cytokinesis.  |  Echard, A. 2008. Biochem Soc Trans. 36: 395-9. PMID: 18481967
  7. Rab14/MACF2 complex regulates endosomal targeting during cytokinesis.  |  Gibieža, P., et al. 2021. Mol Biol Cell. 32: 554-566. PMID: 33566684
  8. The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly.  |  Liu, YY., et al. 2023. J Virol. 97: e0036423. PMID: 37255314
  9. RAB14 promotes epithelial-mesenchymal transition in bladder cancer through autophagy‑dependent AKT signaling pathway.  |  Deng, H., et al. 2023. Cell Death Discov. 9: 292. PMID: 37558664
  10. Biogenesis of Rab14-positive endosome buds at Golgi-endosome contacts by the RhoBTB3-SHIP164-Vps26B complex.  |  Wang, J., et al. 2024. Cell Discov. 10: 38. PMID: 38565878
  11. Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily.  |  Olkkonen, VM., et al. 1993. J Cell Sci. 106 (Pt 4): 1249-61. PMID: 8126105
  12. RAB GTPases expressed in human melanoma cells.  |  Chen, D., et al. 1997. Biochim Biophys Acta. 1355: 1-6. PMID: 9030196

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rab 14 Antibody (D-5)

sc-271401
200 µg/ml
$316.00

Rab 14 Antibody (D-5): m-IgG Fc BP-HRP Bundle

sc-527241
200 µg Ab; 10 µg BP
$354.00

Rab 14 Antibody (D-5): m-IgG1 BP-HRP Bundle

sc-532614
200 µg Ab; 20 µg BP
$354.00

Rab 14 Antibody (D-5) AC

sc-271401 AC
500 µg/ml, 25% agarose
$416.00

Rab 14 Antibody (D-5) HRP

sc-271401 HRP
200 µg/ml
$316.00

Rab 14 Antibody (D-5) FITC

sc-271401 FITC
200 µg/ml
$330.00

Rab 14 Antibody (D-5) PE

sc-271401 PE
200 µg/ml
$343.00

Rab 14 Antibody (D-5) Alexa Fluor® 488

sc-271401 AF488
200 µg/ml
$357.00

Rab 14 Antibody (D-5) Alexa Fluor® 546

sc-271401 AF546
200 µg/ml
$357.00

Rab 14 Antibody (D-5) Alexa Fluor® 594

sc-271401 AF594
200 µg/ml
$357.00

Rab 14 Antibody (D-5) Alexa Fluor® 647

sc-271401 AF647
200 µg/ml
$357.00

Rab 14 Antibody (D-5) Alexa Fluor® 680

sc-271401 AF680
200 µg/ml
$357.00

Rab 14 Antibody (D-5) Alexa Fluor® 790

sc-271401 AF790
200 µg/ml
$357.00

Are there any issues with using sc-271401: Rab 14 (D-5) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271401 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

What would the appropriate isotype control antibody be for this antibody? Mouse IgG1?

Asked by: MichaelC
Thank you for your question. The proper isotype control for our Rab 14 antibody sc-271401 would be a normal mouse IgG1, such as sc-3877 (https://www.scbt.com/scbt/product/normal-mouse-igg1).
Answered by: Technical Service
Date published: 2016-11-30
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Rated 4 out of 5 by from RAB14 AntibodyI used RAB14 coniugated with Goat anti-Mouse IgG, Alexa Fluor® 568 and gave a very specific peak using a 1:50 dilution.
Date published: 2023-01-26
Rated 5 out of 5 by from Powerful signalWe received this antibody as a free trial sample a month ago. Due to the fact that it is a little amount we only assayed one dilution on western blotting (1/500). Signal was great and the band, well defined. We are really happy with the quality of this antibody and we aim to test it on other techniques( IF and FC).
Date published: 2017-10-20
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Rab 14 expression in LADMAC and Hep G2 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from Western Blot of LADMAC and Hep G2 whole cellWestern Blot of LADMAC and Hep G2 whole cell lysates showed very strong bands -SCBT QC
Date published: 2014-09-17
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Rab 14 Antibody (D-5) is rated 4.8 out of 5 by 4.
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