Rab 14 Antibody (D-5): sc-271401
- Rab 14 Antibody (D-5) is a mouse monoclonal IgG1, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 161-215 mapping at the C-terminus of Rab 14 of human origin
- Rab 14 Antibody (D-5) is recommended for detection of Rab 14 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Rab 14 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 14 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 14 Antibody (D-5) (see ordering information below).
Rab 14 Antibody (D-5) is a mouse monoclonal IgG1 antibody that detects Rab 14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rab 14 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 14, also known as FBP, is a 215 amino acid protein that plays a crucial role in vesicular trafficking and neurotransmitter release, making Rab 14 essential for cellular communication and transport processes. Rab 14 is lipid-anchored to the cytoplasmic side of the cell membrane and is predominantly expressed in the brain, lung, kidney, spleen, and thymus, highlighting Rab 14′s importance in various physiological functions. As a member of the Rab subfamily of small GTPases, Rab 14 is integral to the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi complex and secretory vesicles, as well as in endocytic pathways, thereby facilitating the movement of carrier vesicles essential for maintaining cellular homeostasis and signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rab 14 Antibody (D-5) References:
- Intracellular membrane trafficking pathways in bone-resorbing osteoclasts revealed by cloning and subcellular localization studies of small GTP-binding rab proteins. | Zhao, H., et al. 2002. Biochem Biophys Res Commun. 293: 1060-5. PMID: 12051767
- Rab14 is involved in membrane trafficking between the Golgi complex and endosomes. | Junutula, JR., et al. 2004. Mol Biol Cell. 15: 2218-29. PMID: 15004230
- Large scale screening for novel rab effectors reveals unexpected broad Rab binding specificity. | Fukuda, M., et al. 2008. Mol Cell Proteomics. 7: 1031-42. PMID: 18256213
- Annexin A2 interactions with Rab14 in alveolar type II cells. | Gou, D., et al. 2008. J Biol Chem. 283: 13156-64. PMID: 18332131
- Rab14 regulates apical targeting in polarized epithelial cells. | Kitt, KN., et al. 2008. Traffic. 9: 1218-31. PMID: 18429929
- Membrane traffic and polarization of lipid domains during cytokinesis. | Echard, A. 2008. Biochem Soc Trans. 36: 395-9. PMID: 18481967
- Rab14/MACF2 complex regulates endosomal targeting during cytokinesis. | Gibieža, P., et al. 2021. Mol Biol Cell. 32: 554-566. PMID: 33566684
- The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. | Liu, YY., et al. 2023. J Virol. 97: e0036423. PMID: 37255314
- RAB14 promotes epithelial-mesenchymal transition in bladder cancer through autophagy‑dependent AKT signaling pathway. | Deng, H., et al. 2023. Cell Death Discov. 9: 292. PMID: 37558664
- Biogenesis of Rab14-positive endosome buds at Golgi-endosome contacts by the RhoBTB3-SHIP164-Vps26B complex. | Wang, J., et al. 2024. Cell Discov. 10: 38. PMID: 38565878
- Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. | Olkkonen, VM., et al. 1993. J Cell Sci. 106 (Pt 4): 1249-61. PMID: 8126105
- RAB GTPases expressed in human melanoma cells. | Chen, D., et al. 1997. Biochim Biophys Acta. 1355: 1-6. PMID: 9030196
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 14 Antibody (D-5) | sc-271401 | 200 µg/ml | $322.00 | |||
Rab 14 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-527241 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rab 14 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-532614 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rab 14 Antibody (D-5) AC | sc-271401 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rab 14 Antibody (D-5) HRP | sc-271401 HRP | 200 µg/ml | $322.00 | |||
Rab 14 Antibody (D-5) FITC | sc-271401 FITC | 200 µg/ml | $336.00 | |||
Rab 14 Antibody (D-5) PE | sc-271401 PE | 200 µg/ml | $349.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 488 | sc-271401 AF488 | 200 µg/ml | $364.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 546 | sc-271401 AF546 | 200 µg/ml | $364.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 594 | sc-271401 AF594 | 200 µg/ml | $364.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 647 | sc-271401 AF647 | 200 µg/ml | $364.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 680 | sc-271401 AF680 | 200 µg/ml | $364.00 | |||
Rab 14 Antibody (D-5) Alexa Fluor® 790 | sc-271401 AF790 | 200 µg/ml | $364.00 |