Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 12 CRISPR/Cas9 KO Plasmid (h): sc-415015
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 12 Antibody (H-11): sc-515613
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 12 CRISPR/Cas9 KO Plasmid (h) | sc-415015 | 20 µg | $397.00 |
Overview
RAB12 encodes Rab12, a small GTPase that regulates membrane trafficking within the endosomal–lysosomal system. Rab12 contributes to late endosome and lysosome dynamics, influencing cargo sorting, receptor turnover, and autophagy-associated degradation pathways. By modulating intracellular trafficking and proteostasis, Rab12 can affect cellular responses to nutrient status and stress signaling networks. Dysregulated Rab GTPase-dependent transport has been linked to altered metabolism and invasive phenotypes, supporting investigation of RAB12 in disease-relevant cell models.
Rab 12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAB12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAB12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAB12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 12 protein expression.
This CRISPR knockout system enables efficient generation of RAB12-deficient cell models for investigation of Rab 12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAB12 exon(s) critical for Rab 12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAB12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 12 CRISPR/Cas9 KO Plasmid (h) and Rab 12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAB12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 12 HDR Plasmid (h) and Rab 12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAB12 homology arms to support homology-directed repair at defined RAB12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.