Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
QTRT1 CRISPR/Cas9 KO Plasmid (h): sc-413456
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- QTRT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the QTRT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: QTRT1 Antibody (D-7): sc-398918
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QTRT1 CRISPR/Cas9 KO Plasmid (h) | sc-413456 | 20 µg | $397.00 |
Overview
QTRT1 encodes the catalytic subunit of tRNA-guanine transglycosylase, an enzyme required for queuosine modification at the wobble position of specific cytosolic tRNAs. This tRNA editing process supports translational accuracy and efficiency, influencing codon-biased protein synthesis, proteostasis, and broader RNA metabolism. Through its role in maintaining tRNA modification homeostasis, QTRT1 links nutrient-dependent queuine salvage to cellular stress responses and growth control pathways. Dysregulation of tRNA modifications and the translation machinery has been associated with phenotypes relevant to mitochondrial function, oxidative stress sensitivity, and proliferative programs, making QTRT1 a useful node for mechanistic studies of RNA-modification biology.
QTRT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the QTRT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the QTRT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the QTRT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish QTRT1 protein expression.
This CRISPR knockout system enables efficient generation of QTRT1-deficient cell models for investigation of QTRT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting QTRT1 exon(s) critical for QTRT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple QTRT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by QTRT1 CRISPR/Cas9 KO Plasmid (h) and QTRT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the QTRT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by QTRT1 HDR Plasmid (h) and QTRT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by QTRT1 homology arms to support homology-directed repair at defined QTRT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.