Qa-1 Antibody (6A8.6F10): sc-23889

Qa-1 Antibody (6A8.6F10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Qa-1 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-Qa-1 antibody (6A8.6F10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Qa-1 protein, a member of the major histocompatibility complex (MHC) class Ib molecules, plays a crucial role in the immune response by presenting the Qa-1 determinant modifier (Qdm) peptide to the CD94/NKG2A receptor on natural killer (NK) cells. This interaction is vital for maintaining self-tolerance, as NK cell cytotoxicity against self cells is inhibited, thereby preventing autoimmune reactions. Qa-1 protein preferentially binds to CD8+ T cells, which further underscores its importance in modulating immune responses. The gene encoding murine Qa-1 is located on chromosome 17, highlighting its evolutionary significance in the context of immune regulation. Anti-Qa-1 antibody (6A8.6F10) serves as an essential tool for researchers studying immune mechanisms and cellular interactions in mouse models.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Qa-1 Antibody (6A8.6F10) References:

1. Analysis of Qa-1(b) peptide binding specificity and the capacity of CD94/NKG2A to discriminate between Qa-1-peptide complexes.  |  Kraft, JR., et al. 2000. J Exp Med. 192: 613-24. PMID: 10974028
2. The non-classical MHC class I molecule Qa-1(b) inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes.  |  Lohwasser, S., et al. 2001. Int Immunol. 13: 321-7. PMID: 11222501
3. Mouse CD94 participates in Qa-1-mediated self recognition by NK cells and delivers inhibitory signals independent of Ly-49.  |  Toyama-Sorimachi, N., et al. 2001. J Immunol. 166: 3771-9. PMID: 11238619
4. The multiple roles of major histocompatibility complex class-I-like molecules in mucosal immune function.  |  Blumberg, RS., et al. 2001. Acta Odontol Scand. 59: 139-44. PMID: 11501882
5. Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene.  |  Soloski, MJ., et al. 1981. J Exp Med. 153: 1080-93. PMID: 6788884
6. Biochemical analysis of an MHC-linked hematopoietic cell surface antigen, Qa-2.  |  Soloski, MJ., et al. 1981. J Supramol Struct Cell Biochem. 16: 167-77. PMID: 7299842
7. The class I-b molecule Qa-1 forms heterodimers with H-2Ld and a novel 50-kD glycoprotein encoded centromeric to I-E beta.  |  Wolf, PR. and Cook, RG. 1995. J Exp Med. 181: 657-68. PMID: 7836920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Qa-1 Antibody (6A8.6F10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Qa-1 Antibody (6A8.6F10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Qa-1 Antibody (6A8.6F10) Specifications

Alternate Product Name: Qa-1

Product Clone Name: 6A8.6F10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 161-179

Epitope Target: Qa-1

Epitope Species: mouse

Product Reactivity/Detection: non classical MHC Class I (Class Ib) molecule Qa-1

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Qa-1 Antibody (6A8.6F10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin