PYST2 Antibody (D-8): sc-377106

PYST2 Antibody (D-8) is a mouse monoclonal IgM kappa antibody that detects PYST2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PYST2 (D-8) antibody is available as the non-conjugated form. PYST2 protein plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways, which are essential for various cellular processes, including growth, differentiation, and response to stress. PYST2 functions as a dual specificity phosphatase, selectively dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases, thereby inactivating members of the MAPK/ERK pathway. This regulatory mechanism is vital, as dysregulation of MAP kinases can lead to pathological conditions, including cancer. PYST2 is overexpressed in patients with acute myelogenous leukemia (AML), highlighting potential as a biomarker for this disease and suggesting that targeting PYST2 may offer therapeutic avenues for intervention.

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PYST2 Antibody (D-8) References:

1. Dual specificity phosphatases: a gene family for control of MAP kinase function.  |  Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
2. Overexpression of the dual-specificity MAPK phosphatase PYST2 in acute leukemia.  |  Levy-Nissenbaum, O., et al. 2003. Cancer Lett. 199: 185-92. PMID: 12969791
3. Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells.  |  Levy-Nissenbaum, O., et al. 2003. Oncogene. 22: 7649-60. PMID: 14576828
4. cDNA microarray analysis reveals an overexpression of the dual-specificity MAPK phosphatase PYST2 in acute leukemia.  |  Levy-Nissenbaum, O., et al. 2003. Methods Enzymol. 366: 103-13. PMID: 14674243
5. An emerging family of dual specificity MAP kinase phosphatases.  |  Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
6. Isolation of the human genes encoding the pyst1 and Pyst2 phosphatases: characterisation of Pyst2 as a cytosolic dual-specificity MAP kinase phosphatase and its catalytic activation by both MAP and SAP kinases.  |  Dowd, S., et al. 1998. J Cell Sci. 111 (Pt 22): 3389-99. PMID: 9788880
7. Characterization of the dual-specificity phosphatase PYST2 and its transcripts.  |  Levy-Nissenbaum, O., et al. 2004. Genes Chromosomes Cancer. 39: 37-47. PMID: 14603440

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PYST2 Antibody (D-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PYST2 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PYST2 Antibody (D-8) Specifications

Alternate Product Name: PYST2

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 341-368

Epitope Target: PYST2

Epitope Species: human

Product Reactivity/Detection: PYST2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PYST2 Antibody (D-8): P: Blocking Peptide