Pyrin Antibody (C-11): sc-390938

Pyrin Antibody (C-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects Pyrin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pyrin (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pyrin, also known as Marenostrin or Mediterranean fever protein, plays a crucial role in regulating inflammatory response within myelomonocytic cells, particularly influencing cytoskeletal organization. Pyrin′s function is vital in innate immune response, where Pyrin acts as a sensor for cellular stress and infection, leading to activation of inflammatory pathways. Defects in Pyrin gene can result in Mediterranean fever, a hereditary autosomal recessive disorder characterized by recurrent fever and serosal inflammation, highlighting Pyrin′s critical role in maintaining immune homeostasis. Pyrin is predominantly expressed in peripheral blood leukocytes, especially in mature granulocytes, and is absent in lymphocytes, with additional expression noted in spleen, muscle, lung, and various myeloid leukemic, colon cancer, and prostate cancer cell lines.

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Pyrin Antibody (C-11) References:

1. The gene for familial Mediterranean fever, MEFV, is expressed in early leukocyte development and is regulated in response to inflammatory mediators.  |  Centola, M., et al. 2000. Blood. 95: 3223-31. PMID: 10807793
2. Mutations in the MEFV gene in a large series of patients with a clinical diagnosis of familial Mediterranean fever.  |  Dodé, C., et al. 2000. Am J Med Genet. 92: 241-6. PMID: 10842288
3. Alternative splicing at the MEFV locus involved in familial Mediterranean fever regulates translocation of the marenostrin/pyrin protein to the nucleus.  |  Papin, S., et al. 2000. Hum Mol Genet. 9: 3001-9. PMID: 11115844
4. Pyrin binds the PSTPIP1/CD2BP1 protein, defining familial Mediterranean fever and PAPA syndrome as disorders in the same pathway.  |  Shoham, NG., et al. 2003. Proc Natl Acad Sci U S A. 100: 13501-6. PMID: 14595024
5. A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.  |  Aglipay, JA., et al. 2003. Oncogene. 22: 8931-8. PMID: 14654789
6. Apoptotic speck protein-like, a highly homologous protein to apoptotic speck protein in the pyrin domain, is silenced by DNA methylation and induces apoptosis in human hepatocellular carcinoma.  |  Kubo, T., et al. 2004. Cancer Res. 64: 5172-7. PMID: 15289321
7. Role of charged and hydrophobic residues in the oligomerization of the PYRIN domain of ASC.  |  Moriya, M., et al. 2005. Biochemistry. 44: 575-83. PMID: 15641782
8. Pyrin Inflammasome Regulates Tight Junction Integrity to Restrict Colitis and Tumorigenesis.  |  Sharma, D., et al. 2018. Gastroenterology. 154: 948-964.e8. PMID: 29203393
9. The Pyrin Inflammasome in Health and Disease.  |  Schnappauf, O., et al. 2019. Front Immunol. 10: 1745. PMID: 31456795
10. HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation.  |  Magupalli, VG., et al. 2020. Science. 369: PMID: 32943500
11. Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole‑kindled mice.  |  Wang, L., et al. 2021. Int J Mol Med. 48: PMID: 34676876
12. Alpha-linolenic acid pretreatment alleviates NETs-induced alveolar macrophage pyroptosis by inhibiting pyrin inflammasome activation in a mouse model of sepsis-induced ALI/ARDS.  |  Liu, C., et al. 2023. Front Immunol. 14: 1146612. PMID: 37051243

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pyrin Antibody (C-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Pyrin Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pyrin Antibody (C-11) Specifications

Alternate Product Name: Pyrin

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 231-368

Epitope Target: Pyrin

Epitope Species: human

Product Reactivity/Detection: Pyrin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Pyrin Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin