PYPAF7 Antibody (A-3): sc-390666

PYPAF7 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PYPAF7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PYPAF7 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PYPAF7, also known as Monarch-1, plays a crucial role in regulating inflammatory responses and apoptosis by modulating caspase-1 activation through ASC protein and promoting NFκB activation via IKK. This regulation is vital for maintaining immune homeostasis and responding to infections, as dysregulation of these pathways can lead to inflammatory diseases. PYPAF7 is predominantly located in cytoplasm of peripheral blood leukocytes, particularly in eosinophils and granulocytes, with lower expression levels in monocytes. PYPAF7 gene is mapped to human chromosome 19q13.4, highlighting evolutionary conservation and significance across species.

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PYPAF7 Antibody (A-3) References:

1. PYPAF7, a novel PYRIN-containing Apaf1-like protein that regulates activation of NF-kappa B and caspase-1-dependent cytokine processing.  |  Wang, L., et al. 2002. J Biol Chem. 277: 29874-80. PMID: 12019269
2. Functional screening of five PYPAF family members identifies PYPAF5 as a novel regulator of NF-kappaB and caspase-1.  |  Grenier, JM., et al. 2002. FEBS Lett. 530: 73-8. PMID: 12387869
3. Cutting edge: Monarch-1: a pyrin/nucleotide-binding domain/leucine-rich repeat protein that controls classical and nonclassical MHC class I genes.  |  Williams, KL., et al. 2003. J Immunol. 170: 5354-8. PMID: 12759408
4. Asc-mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP.  |  Hasegawa, M., et al. 2005. J Biol Chem. 280: 15122-30. PMID: 15701651
5. PYPAF3, a PYRIN-containing APAF-1-like protein, is a feedback regulator of caspase-1-dependent interleukin-1beta secretion.  |  Kinoshita, T., et al. 2005. J Biol Chem. 280: 21720-5. PMID: 15817483
6. The CATERPILLER protein monarch-1 is an antagonist of toll-like receptor-, tumor necrosis factor alpha-, and Mycobacterium tuberculosis-induced pro-inflammatory signals.  |  Williams, KL., et al. 2005. J Biol Chem. 280: 39914-24. PMID: 16203735
7. Exposure in vivo to silica or lipopolysaccharide produces transient or sustained upregulation, respectively, of PYPAF7 and MEFV genes in bronchoalveolar lavage cells in rats.  |  Rao, KM. and Meighan, T. 2006. J Toxicol Environ Health A. 69: 481-90. PMID: 16574623
8. Monarch-1 suppresses non-canonical NF-kappaB activation and p52-dependent chemokine expression in monocytes.  |  Lich, JD., et al. 2007. J Immunol. 178: 1256-60. PMID: 17237370
9. Monarch-1/PYPAF7 and other CATERPILLER (CLR, NOD, NLR) proteins with negative regulatory functions.  |  Lich, JD. and Ting, JP. 2007. Microbes Infect. 9: 672-6. PMID: 17418609
10. Inflammasome mRNA expression in human monocytes during early septic shock.  |  Fahy, RJ., et al. 2008. Am J Respir Crit Care Med. 177: 983-8. PMID: 18263805
11. The multifaceted nature of NLRP12.  |  Tuncer, S., et al. 2014. J Leukoc Biol. 96: 991-1000. PMID: 25249449
12. MONARCH 1, A Phase II Study of Abemaciclib, a CDK4 and CDK6 Inhibitor, as a Single Agent, in Patients with Refractory HR+/HER2- Metastatic Breast Cancer.  |  Dickler, MN., et al. 2017. Clin Cancer Res. 23: 5218-5224. PMID: 28533223

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PYPAF7 Antibody (A-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PYPAF7 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PYPAF7 Antibody (A-3) Specifications

Alternate Product Name: PYPAF7

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 853-892

Epitope Target: PYPAF7

Epitope Species: mouse

Product Reactivity/Detection: PYPAF7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PYPAF7 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin