Pumilio 2 Antibody (C-8): sc-514108

Pumilio 2 Antibody (C-8) is a mouse monoclonal IgM antibody that detects Pumilio 2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pumilio 2 antibody (C-8) is available as a non-conjugated format. Pumilio 2 is a sequence-specific RNA-binding protein that plays a crucial role in regulating translation and mRNA stability by binding to specific mRNA targets, which is essential for maintaining proper cellular functions. This regulation supports the proliferation and self-renewal of stem cells by controlling the translation of key transcripts necessary for development. The Pumilio gene family is vital for the development of germ stem cells in both sexes, highlighting its importance in reproductive biology. Notably, Pumilio 2 interacts with the human Nanos1 protein, suggesting a conserved mechanism in germ cell development across species. Pumilio 2 is predominantly expressed in the testis and ovary, with lower expression levels found in the brain, heart, kidney, liver, muscle, placenta, intestine, and stomach, as well as in stem cells, germ cells, and most fetal tissues, underscoring its significance in various biological processes.

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Pumilio 2 Antibody (C-8) References:

1. Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins.  |  Spassov, DS. and Jurecic, R. 2002. Gene. 299: 195-204. PMID: 12459267
2. Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins.  |  Moore, FL., et al. 2003. Proc Natl Acad Sci U S A. 100: 538-43. PMID: 12511597
3. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.  |  Jaruzelska, J., et al. 2003. Dev Genes Evol. 213: 120-6. PMID: 12690449
4. Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.  |  Padmanabhan, K. and Richter, JD. 2006. Genes Dev. 20: 199-209. PMID: 16418484
5. Dendritic localization of the translational repressor Pumilio 2 and its contribution to dendritic stress granules.  |  Vessey, JP., et al. 2006. J Neurosci. 26: 6496-508. PMID: 16775137
6. Candidate mRNAs interacting with fertility protein PUMILIO2 in the human germ line.  |  Spik, A., et al. 2006. Reprod Biol. 6 Suppl 1: 37-42. PMID: 16967088
7. Human fertility protein PUMILIO2 interacts in vitro with testis mRNA encoding Cdc42 effector 3 (CEP3).  |  Spik, A., et al. 2006. Reprod Biol. 6: 103-13. PMID: 17173092
8. The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.  |  Ginter-Matuszewska, B., et al. 2009. Mol Hum Reprod. 15: 173-9. PMID: 19168546
9. PUMILIO-2 is involved in the positive regulation of cellular proliferation in human adipose-derived stem cells.  |  Shigunov, P., et al. 2012. Stem Cells Dev. 21: 217-27. PMID: 21649561
10. Translational regulation of acetylcholinesterase by the RNA-binding protein Pumilio-2 at the neuromuscular synapse.  |  Marrero, E., et al. 2011. J Biol Chem. 286: 36492-9. PMID: 21865157
11. Reduced Pumilio-2 expression in patients with temporal lobe epilepsy and in the lithium-pilocarpine induced epilepsy rat model.  |  Wu, XL., et al. 2015. Epilepsy Behav. 50: 31-9. PMID: 26101106
12. RNA binding protein Pumilio2 promotes chemoresistance of pancreatic cancer via focal adhesion pathway and interacting with transcription factor EGR1.  |  Bangbo, Z., et al. 2025. Cell Mol Life Sci. 82: 78. PMID: 39961821

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pumilio 2 Antibody (C-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Pumilio 2 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pumilio 2 Antibody (C-8) Specifications

Alternate Product Name: Pumilio 2

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 153-237

Epitope Target: Pumilio 2

Epitope Species: human

Product Reactivity/Detection: Pumilio 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Pumilio 2 Antibody (C-8): N/A