Pumilio 2 Antibody (A-10): sc-398632
- Pumilio 2 Antibody (A-10) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 102-133 near the N-terminus of Pumilio 2 of human origin
- recommended for detection of Pumilio 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for Pumilio 2 Antibody (A-10) for WB applications. This reagent is now offered in a bundle with Pumilio 2 Antibody (A-10) (see ordering information below).
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Pumilio 2 Antibody (A-10) is a mouse monoclonal IgG3 antibody that detects Pumilio 2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pumilio 2 is a sequence-specific RNA-binding protein that plays a crucial role in regulating translation and mRNA stability by binding to specific mRNA targets, which is essential for maintaining the balance of protein synthesis in cells. This regulation is particularly important in stem cells, where Pumilio 2 supports proliferation and self-renewal by controlling the translation of key transcripts necessary for development. The Pumilio gene encodes proteins that are vital for the development of germ stem cells in one or both sexes, and Pumilio 2 has been shown to interact with the human Nanos1 protein, suggesting a conserved role in germ cell development across species. Notably, Pumilio 2 is highly expressed in the testis and ovary, with lower expression levels found in the brain, heart, kidney, liver, muscle, placenta, intestine, and stomach, as well as in stem cells, germ cells, and most fetal tissues, highlighting its significance in reproductive biology and developmental processes.
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Pumilio 2 Antibody (A-10) References:
- Polymorphisms of the human PUMILIO2 gene and male sterility. | Kusz, K., et al. 2007. Mol Reprod Dev. 74: 795-9. PMID: 17154300
- NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells. | Ginter-Matuszewska, B., et al. 2011. Histochem Cell Biol. 136: 279-87. PMID: 21800163
- Pumilio-2 function in the mouse nervous system. | Siemen, H., et al. 2011. PLoS One. 6: e25932. PMID: 22016787
- Pumilio-2 regulates translation of Nav1.6 to mediate homeostasis of membrane excitability. | Driscoll, HE., et al. 2013. J Neurosci. 33: 9644-54. PMID: 23739961
- MiR-134-dependent regulation of Pumilio-2 is necessary for homeostatic synaptic depression. | Fiore, R., et al. 2014. EMBO J. 33: 2231-46. PMID: 25097251
- Pumilio2-deficient mice show a predisposition for epilepsy. | Follwaczny, P., et al. 2017. Dis Model Mech. 10: 1333-1342. PMID: 29046322
- Pumilio2 regulates synaptic plasticity via translational repression of synaptic receptors in mice. | Dong, H., et al. 2018. Oncotarget. 9: 32134-32148. PMID: 30181804
- Effects of PUMILIO1 and PUMILIO2 knockdown on cardiomyogenic differentiation of human embryonic stem cells culture. | Silva, ILZ., et al. 2020. PLoS One. 15: e0222373. PMID: 32437472
- Pumilio2 and Staufen2 selectively balance the synaptic proteome. | Schieweck, R., et al. 2021. Cell Rep. 35: 109279. PMID: 34161769
- Pumilio2 Promotes Growth of Mature Neurons. | Schieweck, R., et al. 2021. Int J Mol Sci. 22: PMID: 34445704
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pumilio 2 Antibody (A-10) | sc-398632 | 200 µg/ml | $322.00 | |||
Pumilio 2 Antibody (A-10): m-IgG3 BP-HRP Bundle | sc-550510 | 200 µg Ab; 40 µg BP | $361.00 | |||
Pumilio 2 (A-10) Neutralizing Peptide | sc-398632 P | 100 µg/0.5 ml | $69.00 |