Date published: 2025-12-8

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PTTG Antibody (3F313): sc-71947

5.0(2)
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Datasheets
  • PTTG Antibody (3F313) is a mouse monoclonal IgG2a, cited in 2 publications, provided at 200 µg/ml
  • raised against full length PTTG of human origin
  • recommended for detection of PTTG of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See PTTG (DCS-280): sc-56207 for PTTG antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG2a BP-HRP is the preferred secondary detection reagent for PTTG Antibody (3F313) for WB and IHC(P) applications. This reagent is now offered in a bundle with PTTG Antibody (3F313) (see ordering information below).

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PTTG Antibody (3F313) is a mouse monoclonal IgG2a antibody that detects pituitary tumor transforming gene (PTTG) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PTTG plays a crucial role in cell cycle regulation by acting as an anaphase inhibitor, specifically regulating separin Esp1, which is essential for chromatid separation during mitosis. This function helps ensure proper chromosome segregation, thereby preventing chromosomal instability, a hallmark of many cancers. PTTG expression has been observed in various human tissues, including thymus, testis, and placenta, with primary localization in cytoplasm and partial presence in nucleus, underscoring PTTG′s importance in cellular processes. PTTG′s interaction with other proteins involved in cell cycle regulation highlights anti-PTTG antibody (3F313) as an invaluable tool for scientists studying tumorigenesis and chromosomal dynamics.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PTTG Antibody (3F313)

sc-71947
200 µg/ml
$316.00

PTTG Antibody (3F313): m-IgG2a BP-HRP Bundle

sc-546005
200 µg Ab; 10 µg BP
$354.00

What is the recommended starting dilution for Western blot for PTTG (3F313): sc-71947 monoclonal antibody?

Asked by: Cweed
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-71947.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very goodVery good antibody. It works for WB of human, mouse, rat cell lines, and also works for IHC of human and mouse specimen slides
Date published: 2017-02-19
Rated 5 out of 5 by from WB publishable data with human HCTWB publishable data with human HCT-116 cells - SCBT Publication Review
Date published: 2014-05-20
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PTTG Antibody (3F313) is rated 5.0 out of 5 by 2.
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