PTRF Antibody (4a): sc-517589

PTRF Antibody (4a) is a mouse monoclonal IgG1 kappa light chain antibody that detects PTRF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-PTRF antibody (4a) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). PTRF protein, also known as Polymerase I and transcript release factor or FKSG13, plays a crucial role in the termination of transcription by RNA polymerase I (Pol I). PTRF monoclonal antibody (4a) is essential for the dissociation of the transcription complex, which is a vital step in the synthesis of ribosomal RNA. This 390 amino acid protein binds to the 3′ end of pre-rRNA while interacting with Pol I and TTF-1 (Thyroid transcription factor-1), facilitating the release of the Pol I complex from the DNA template. Proper functioning of PTRF is critical for maintaining the efficiency of ribosome biogenesis, which is fundamental for cellular growth and proliferation. PTRF (4a) antibody is localized in various cellular compartments, including the cell membrane, microsome, nucleus, and cytoplasm, highlighting its versatile role in cellular processes. Three isoforms of PTRF are produced through alternative splicing, which may contribute to its functional diversity and regulatory mechanisms within different cellular contexts.

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PTRF Antibody (4a) References:

1. PTRF (polymerase I and transcript-release factor) is tissue-specific and interacts with the BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription factor which binds to the two mouse type-I collagen gene promoters.  |  Hasegawa, T., et al. 2000. Biochem J. 347 Pt 1: 55-9. PMID: 10727401
2. The transcript release factor PTRF augments ribosomal gene transcription by facilitating reinitiation of RNA polymerase I.  |  Jansa, P., et al. 2001. Nucleic Acids Res. 29: 423-9. PMID: 11139612
3. Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes.  |  Aboulaich, N., et al. 2004. Biochem J. 383: 237-48. PMID: 15242332
4. Identification of a major protein on the cytosolic face of caveolae.  |  Vinten, J., et al. 2005. Biochim Biophys Acta. 1717: 34-40. PMID: 16236245
5. Association and insulin regulated translocation of hormone-sensitive lipase with PTRF.  |  Aboulaich, N., et al. 2006. Biochem Biophys Res Commun. 350: 657-61. PMID: 17026959
6. PTRF triggers a cave in.  |  Chadda, R. and Mayor, S. 2008. Cell. 132: 23-4. PMID: 18191216
7. PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function.  |  Hill, MM., et al. 2008. Cell. 132: 113-24. PMID: 18191225
8. Identification of a transcript release activity acting on ternary transcription complexes containing murine RNA polymerase I.  |  Mason, SW., et al. 1997. EMBO J. 16: 163-72. PMID: 9009277
9. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes.  |  Jansa, P., et al. 1998. EMBO J. 17: 2855-64. PMID: 9582279

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PTRF Antibody (4a) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PTRF Antibody (4a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PTRF Antibody (4a) Specifications

Alternate Product Name: PTRF

Product Clone Name: 4a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 157-272

Epitope Target: PTRF

Epitope Species: mouse

Product Reactivity/Detection: PTRF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PTRF Antibody (4a): AC: Agarose, HRP: Horseradish Peroxidase