Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PTN CRISPR/Cas9 KO Plasmid (m2): sc-422498-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTN CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PTN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PTN Antibody (H-6): sc-74443
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTN CRISPR/Cas9 KO Plasmid (m2) | sc-422498-KO-2 | 20 µg | $397.00 |
Overview
Ptn encodes pleiotrophin (PTN), a secreted heparin-binding growth factor that modulates cell–cell and cell–matrix communication during development and tissue remodeling. PTN signals through interactions with receptor protein tyrosine phosphatases and other surface partners to influence phosphorylation cascades, neurite outgrowth, cell migration, and survival programs, including MAPK/ERK- and PI3K/AKT-linked processes. In mouse models, PTN is implicated in neurodevelopmental patterning, angiogenic responses, and regulation of inflammatory microenvironments, with altered expression reported across contexts of injury and proliferative signaling. These functions make Ptn a useful node for dissecting extracellular growth factor inputs that shape lineage behavior, synaptic connectivity, and stromal–immune crosstalk.
PTN CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Ptn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ptn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ptn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PTN protein expression.
This CRISPR knockout system enables efficient generation of Ptn-deficient cell models for investigation of PTN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ptn exon(s) critical for PTN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ptn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PTN CRISPR/Cas9 KO Plasmid (m) and PTN CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ptn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PTN HDR Plasmid (m) and PTN HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ptn homology arms to support homology-directed repair at defined Ptn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.