PTN Antibody (H-6) recommended for detection of precursor and mature PTN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PTN Antibody (H-6): sc-74443

PTN Antibody (H-6) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-PTN Antibody (H-6) is a mouse monoclonal IgG1 (kappa light chain) PTN antibody provided at 200 µg/ml
    • raised against amino acids 94-168 of PTN of human origin
    • Anti-PTN Antibody (H-6) is recommended for detection of precursor and mature PTN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PTN Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PTN (H-6): sc-74443.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Hi I am using this antibody for western blot in NT2 cells and I detect two bands around molecular weight 25KDa and 17KDa. I also noticed you have shown the western blot image of PTN in NT2 cells. Could you help us verify which is the correct PTN band.

Asked by: Charan
Thank you for your question. PTN has a predicted molecular weight of ~19 kDa, however we typically observe PTN in cell lines between 18-25 kDa. The Western blot data published on our website is representative of what we observe using the antibody in cell lines tested. We recommend you include positive and negative controls in your evaluation of the antibody in your samples to evaluate the specificity of your results. Please contact our Technical Service Department for support in troubleshooting the antibody.
Answered by: Technical Support
Date published: 2019-06-07

I am using PTN (H-6): sc-74443 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great choice for WB Produced robust, clean WB bands in CI-H460, NTERA-2 cl.D1 and NIH/3T3 whole cell lysates.
Date published: 2017-01-30
Rated 5 out of 5 by from Published IF data using Human amniotic epithelial Published IF data using Human amniotic epithelial cells. -SCBT Publication Review
Date published: 2015-06-18
Rated 5 out of 5 by from Robust signal in western blot analysis of Robust signal in western blot analysis of human recombinant PTN under reducing conditions. -SCBT QC
Date published: 2015-06-15
Rated 5 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human parathyroid gland tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Published immunohistochemical analysis using Published immunohistochemical analysis using paraffin embedded endothelial cells. -SCBT Publication Review
Date published: 2015-01-12
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