PTN Antibody (H-6): sc-74443

PTN Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PTN protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PTN antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pleiotrophin (PTN) is a heparin-binding growth factor that plays a crucial role in cellular processes such as cell proliferation, differentiation, and migration, making PTN significant in developmental biology and tissue repair. PTN protein is synthesized as a 168 amino acid precursor that is cleaved to yield a 136 amino acid active form, exhibiting approximately 50% identity with midkine (MK) and maintaining conservation of all ten cysteine residues, which are essential for structural integrity and function. PTN is predominantly expressed in various cell types, including osteoblasts, chondrocytes, and neurons, and is particularly important during fetal development, where PTN supports the growth and differentiation of neural tissues. In adult organisms, PTN expression is largely restricted to the brain, highlighting PTN′s specialized role in neurodevelopment and potential implications in neurodegenerative diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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PTN Antibody (H-6) References:

1. Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues.  |  Vanderwinden, JM., et al. 1992. Anat Embryol (Berl). 186: 387-406. PMID: 1416088
2. Cloning, nucleotide sequence, and chromosome localization of the human pleiotrophin gene.  |  Milner, PG., et al. 1992. Biochemistry. 31: 12023-8. PMID: 1457401
3. Characterization of the human pleiotrophin gene. Promoter region and chromosomal localization.  |  Li, YS., et al. 1992. J Biol Chem. 267: 26011-6. PMID: 1464612
4. Expression of the HBNF (heparin-binding neurite-promoting factor) gene in the brain of fetal, neonatal and adult rat: an in situ hybridization study.  |  Bloch, B., et al. 1992. Brain Res Dev Brain Res. 70: 267-78. PMID: 1477961
5. HBNF and MK, members of a novel gene family of heparin-binding proteins with potential roles in embryogenesis and brain function.  |  Böhlen, P. and Kovesdi, I. 1991. Prog Growth Factor Res. 3: 143-57. PMID: 1773041
6. A new heparin binding protein regulated by retinoic acid from chick embryo.  |  Raulais, D., et al. 1991. Biochem Biophys Res Commun. 174: 708-15. PMID: 1993066
7. Cloning and expression of a developmentally regulated protein that induces mitogenic and neurite outgrowth activity.  |  Li, YS., et al. 1990. Science. 250: 1690-4. PMID: 2270483
8. Pericyte loss leads to circulatory failure and pleiotrophin depletion causing neuron loss.  |  Nikolakopoulou, AM., et al. 2019. Nat Neurosci. 22: 1089-1098. PMID: 31235908
9. Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.  |  Liu, Z., et al. 2022. J Clin Invest. 132: PMID: 35143418
10. Pleiotrophin ameliorates age-induced adult hippocampal neurogenesis decline and cognitive dysfunction.  |  Li, H., et al. 2023. Cell Rep. 42: 113022. PMID: 37610873
11. Pleiotrophin transforms NIH 3T3 cells and induces tumors in nude mice.  |  Chauhan, AK., et al. 1993. Proc Natl Acad Sci U S A. 90: 679-82. PMID: 8421705
12. Developmentally regulated expression of pleiotrophin, a novel heparin binding growth factor, in the nervous system of the rat.  |  Wanaka, A., et al. 1993. Brain Res Dev Brain Res. 72: 133-44. PMID: 8453763

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PTN Antibody (H-6) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-PTN Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PTN Antibody (H-6) Specifications

Alternate Product Name: PTN

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 94-168

Epitope Target: PTN

Epitope Species: human

Product Reactivity/Detection: precursor and mature PTN

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for PTN Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin