PSTPIP1 Antibody (B-10): sc-390727

PSTPIP1 Antibody (B-10) is a mouse monoclonal IgG2a antibody that detects PSTPIP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PSTPIP1 antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSTPIP1, also known as proline-serine-threonine phosphatase interacting protein 1, H-PIP, PAPAS, CD2BP1, CD2BP1L, or CD2BP1S, consists of 416 amino acids and is primarily localized in the cytoplasm and cytoskeleton, where PSTPIP1 plays a crucial role in the regulation of the actin cytoskeleton. PSTPIP1 contains one SH3 domain and one FCH domain, which are essential for interactions with other proteins, including CD2AP, BDP1, and c-Abl. These interactions suggest PSTPIP1 may function as a scaffold protein, promoting actin polymerization, which is vital for various cellular processes such as cell motility, shape maintenance, and intracellular transport. Notably, defects in the gene encoding PSTPIP1 are linked to PAPA syndrome, an autosomal dominant condition characterized by recurrent inflammatory episodes affecting the skin and joints. Additionally, multiple isoforms of PSTPIP1 arise from alternative splicing, contributing to functional diversity in different cellular contexts.

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PSTPIP1 Antibody (B-10) References:

1. Cytoskeletal protein PSTPIP1 directs the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation.  |  Cong, F., et al. 2000. Mol Cell. 6: 1413-23. PMID: 11163214
2. PSTPIP is a substrate of PTP-PEST and serves as a scaffold guiding PTP-PEST toward a specific dephosphorylation of WASP.  |  Côté, JF., et al. 2002. J Biol Chem. 277: 2973-86. PMID: 11711533
3. Mutations in CD2BP1 disrupt binding to PTP PEST and are responsible for PAPA syndrome, an autoinflammatory disorder.  |  Wise, CA., et al. 2002. Hum Mol Genet. 11: 961-9. PMID: 11971877
4. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants.  |  Yu, JW., et al. 2007. Mol Cell. 28: 214-27. PMID: 17964261
5. PSTPIP: a tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for a PEST tyrosine phosphatase.  |  Spencer, S., et al. 1997. J Cell Biol. 138: 845-60. PMID: 9265651
6. Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein.  |  Dowbenko, D., et al. 1998. J Biol Chem. 273: 989-96. PMID: 9422760
7. Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein.  |  Wu, Y., et al. 1998. J Biol Chem. 273: 5765-70. PMID: 9488710
8. A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion.  |  Li, J., et al. 1998. EMBO J. 17: 7320-36. PMID: 9857189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSTPIP1 Antibody (B-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PSTPIP1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSTPIP1 Antibody (B-10) Specifications

Alternate Product Name: PSTPIP1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 1-94

Epitope Target: PSTPIP1

Epitope Species: human

Product Reactivity/Detection: PSTPIP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PSTPIP1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin