PSR Antibody (C-1): sc-48405

PSR Antibody (C-1) is a mouse monoclonal IgG1 antibody that detects phosphatidylserine receptor (PSR) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PSR (C-1) antibody plays a crucial role in the recognition and clearance of apoptotic cells, as PSR monoclonal antibody (C-1) specifically binds to phosphatidylserine, which becomes exposed on the outer membrane of cells undergoing apoptosis. This interaction is vital for the phagocytosis of dying cells by immune cells like macrophages and dendritic cells, thereby maintaining tissue homeostasis and preventing inflammation. PSR is predominantly expressed on the surface of macrophages, fibroblasts, and epithelial cells, with particularly high levels found in heart, skeletal muscle, and kidney tissues. Notably, PSR includes extensive glycosylation, which is important for stability and function, as glycosylation can influence protein folding, receptor-ligand interactions, and immune recognition. Additionally, mammalian PSR shows significant homology to proteins found in Caenorhabditis elegans and Drosophila melanogaster, indicating that essential functions have been conserved throughout evolution.

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PSR Antibody (C-1) References:

1. Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms: a process upregulated by dexamethasone.  |  Sexton, DW., et al. 2001. J Allergy Clin Immunol. 108: 962-9. PMID: 11742274
2. Activation of protein kinase C beta II by the stereo-specific phosphatidylserine receptor is required for phagocytosis of apoptotic thymocytes by resident murine tissue macrophages.  |  Todt, JC., et al. 2002. J Biol Chem. 277: 35906-14. PMID: 12114511
3. Clearance of apoptotic photoreceptors: elimination of apoptotic debris into the subretinal space and macrophage-mediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3.  |  Hisatomi, T., et al. 2003. Am J Pathol. 162: 1869-79. PMID: 12759244
4. Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells.  |  Fadok, VA., et al. 1992. J Immunol. 149: 4029-35. PMID: 1281199
5. SiMCAL 1 algorithm for analysis of gene expression data related to the phosphatidylserine receptor.  |  Dvorkin, D., et al. 2005. Artif Intell Med. 35: 49-60. PMID: 16099148
6. Changing story of the receptor for phosphatidylserine-dependent clearance of apoptotic cells.  |  Wolf, A., et al. 2007. EMBO Rep. 8: 465-9. PMID: 17471263
7. A lysine-rich motif in the phosphatidylserine receptor PSR-1 mediates recognition and removal of apoptotic cells.  |  Yang, H., et al. 2015. Nat Commun. 6: 5717. PMID: 25564762
8. Reprogramming of cancer-associated fibroblasts by apoptotic cancer cells inhibits lung metastasis via Notch1-WISP-1 signaling.  |  Kim, HJ., et al. 2022. Cell Mol Immunol. 19: 1373-1391. PMID: 36241874
9. Microglial phagocytosis of single dying oligodendrocytes is mediated by CX3CR1 but not MERTK.  |  Olveda, GE., et al. 2024. Cell Rep. 43: 114385. PMID: 38935500
10. CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3).  |  Fadok, VA., et al. 1998. J Immunol. 161: 6250-7. PMID: 9834113

The preferred PSR antibody is PSR Antibody (H-7): sc-28348.